In:
The Journal of Immunology, The American Association of Immunologists, Vol. 188, No. 1_Supplement ( 2012-05-01), p. 120.5-120.5
Abstract:
To investigate whether MyD88 in the gut epithelial brush border plays a role in obesity and metabolic syndrome, we studied a transgenic mouse line expressing MyD88 only in villi (VillinMyD88+/+MyD88-/- mice). In this study, we fed young male wild type B6 (n=8), MyD88-/-B6 (n=6) and VillinMyD88+/+MyD88-/-B6 mice (n=13) with high fat diet (HFD) for 16 weeks. We investigated the metabolic, immunological and gut bacteria profiles of these mice. We found that VillinMyD88+/+MyD88-/-B6 mice had lower body weight, net weight gain, food consumption, total body fat mass, and white adipose tissue weight compared to B6 and/or MyD88-/-B6 mice (P & lt;0.05). Glucose and insulin tolerance tests showed that VillinMyD88+/+MyD88-/-B6 mice were more insulin sensitive than B6 and MyD88-/- B6 mice (P & lt;0.05). Interestingly, white adipose tissue in VillinMyD88+/+MyD88-/-B6 mice harbored fewer B cells, Th17 cells and IL-6+ cells than B6 and/or MyD88-/-B6 mice (P & lt;0.05). Moreover, the number of CD103+CD8+ cells were lower in spleen, mesenteric lymph node, and Peyer Patches of VillinMyD88+/+MyD88-/- B6 mice (P & lt;0.05) compared with B6 and MyD88-/-B6 mice. Gut flora study showed that B6 mice had more Lactobacillus, while MyD88-/-B6 and VillinMyD88+/+MyD88-/-B6 mice had more Enterococcus. However, VillinMyD88+/+MyD88-/-B6 mice had a higher ratio of Gram+/Gram- than MyD88-/-B6 mice in faeces. Our data revealed a novel role of MyD88 in gut epithelial brush border, which affects obesity and metabolism.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.188.Supp.120.5
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2012
detail.hit.zdb_id:
1475085-5
