In:
The Journal of Immunology, The American Association of Immunologists, Vol. 192, No. 1_Supplement ( 2014-05-01), p. 43.8-43.8
Kurzfassung:
Class I Human Leukocyte Antigens distinguish healthy cells from infected cells by presenting peptides at the cell surface. Viral epitopes confirmed as unique to infected cells can lead to successful development of vaccines and therapeutics, and a tool to validate viral epitope presentation on a variety of cell lineages is essential. Here, comparative mass spectrometry shows that the West Nile virus peptide epitopes SVGGVFTSV and ILRNPGYAL are presented by HLA-A*02:01 and HLA-B*07:02 of infected cells. To generate monoclonal antibodies against these HLA/WNV peptide epitopes, mice were immunized with peptide/HLA complexes, splenocytes were fused to myeloma cells, and single clones were picked and grown. Hybridoma supernatants were screened for recognition of the appropriate HLA/WNV peptide complex on peptide-pulsed cells with irrelevant HLA/peptides acting as a negative control. The T cell receptor mimic (TCRm) monoclonal antibodies RL15A and RL29A were found to be specific for A*02:01/SVG9 and B*07:02/ILR9, respectively. The RL15A and RL29A T cell receptor mimic mAb were then used to track viral epitope presentation on WNV infected cell lines and primary cells. In summary, we demonstrate the implementation of a mass spectrometry system for the direct discovery of class I HLA-presented viral epitopes from infected cells followed by the complementary use of TCRm mAb as a companion diagnostic for tracking epitope presentation during the course of a viral infection.
Materialart:
Online-Ressource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.192.Supp.43.8
Sprache:
Englisch
Verlag:
The American Association of Immunologists
Publikationsdatum:
2014
ZDB Id:
1475085-5
