In:
The Journal of Immunology, The American Association of Immunologists, Vol. 194, No. 1_Supplement ( 2015-05-01), p. 65.24-65.24
Abstract:
The pathogenesis of inflammatory skin disease involves interactions between Th17-mediated immune response and keratinocytes. Immersion in pools of thermal mineral water is often considered to have chemical, thermal, mechanical, and immune modulatory benefits to inflammatory skin disease. Therefore, we questioned the effect of thermal treatment on IL-17-mediated immune response in keratinocytes. We evaluated the effect of HSP90 inhibitor on IL-17-mediated cytokine and antimicrobial peptide expression in keratinocytes after heat treatment including IL-1β. To examine the effect of IL-17 receptor-mediated expression of antimicrobial peptides or inflammatory chemokines in HaCaT cells, Act1 siRNA-transfected HaCaT cells were heat stimulated at 42oC and 1 uM of 17-allylaminogeldanamycin (17-AAG) was added. At 37oC, Act1 knockdown decreased BD-1, LL-37, S100A8, S100A9, CXCL1, CXCL5, and CXCL8 expression; however, expression of these antimicrobial peptides and chemokines increased after incubation at 42oC. This thermal-mediated effect was suppressed upon treatment with 17-AAG. We also found that IL-1β expression decreased by 17-AAG after heat stimulation. Although HSP90 enhanced the expression of antimicrobial peptides or chemokines in keratinocytes after heat treatment, HSP90 inhibitors reversed these effects. Therefore, HSP90 inhibitors after heat stimulation (e.g., thermal baths) may be a beneficial therapeutic approach for IL-17-mediated inflammatory skin disease.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.194.Supp.65.24
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2015
detail.hit.zdb_id:
1475085-5
