In:
The Journal of Immunology, The American Association of Immunologists, Vol. 202, No. 1_Supplement ( 2019-05-01), p. 125.10-125.10
Abstract:
Despite treatment and cure, Tuberculosis (TB) is still considered a world health public problem. It is known that interaction between bacteria and host immune mechanisms results in changes in gene expression, which could be a result of epigenetic changes, as DNA methylation. Recent studies suggest that alterations in host epigenome could be promoted by Mtb infection and influence immune cells function. In this context, we aimed to determine the methylation profile of monocytes from tuberculosis patients and correlate with immune function of these cells. Monocytes were isolated from active TB patients (APTB) with treatment-time less than one month (n=10) and non-disease controls (CTRL) (n=16) - Ethics Committee approval FMRP-USP (Protocol #6481/2013). The monocytes were used to evaluate the global DNA methylation content and immune function, through cytokine and ROS production after heat killed Mtb challenge. When we evaluated the group of tuberculosis patients according to their lung injury degree, we found an increased methylation in those with more severe disease. Also, it was possible to observe that monocytes from patients with increased methylation profile, presented a reduction in secretion of anti-inflammatory cytokine, IL-10 and increased pro-inflammatory IL-12, indicating impairment in monocytes ability to regulate the excess of inflammation, which mediates the lung injury often observed in these patients. Hence, we suggested that global DNA methylation content might act as a clinic prognostic toll for active tuberculosis disease.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.202.Supp.125.10
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2019
detail.hit.zdb_id:
1475085-5
