In:
Israeli Journal of Aquaculture - Bamidgeh, SAABRON PRESS, Vol. 74 ( 2022-8-15)
Kurzfassung:
Metapenaeus affinis is a crustacean with important commercial value in the fishery of the South China Sea. Overfishing has resulted in the decline of the wild population and germplasm degradation. However, there is little background knowledge about its gonadal development, and there is a lack of research on the development of this species. To better understand the molecular regulatory mechanisms during gonadal development, here, we performed RNA-Seq on immature and mature ovaries and compared their transcriptomic signatures. 126,930,488 and 122,677,356 clean sequencing reads were obtained from the Illumina sequencing platform, respectively. 394 differentially expressed genes (DEGs) were identified, of which 136 were up-regulated, and 258 were down-regulated. Further analysis revealed rich transcriptional sequences, which have homology with genes related to reproduction and development. Expression patterns of COX, GPX, E3s, PCNA, STPK, and other genes were changed during ovarian development. Validation by qRT-PCR demonstrated the reliability of RNA-Seq. This study has made a significant contribution to the currently available sequence data of M. affinis and provided reference data for the development of genetic and breeding work.
Materialart:
Online-Ressource
ISSN:
0792-156X
,
0792-156X
Sprache:
Englisch
Verlag:
SAABRON PRESS
Publikationsdatum:
2022
ZDB Id:
291787-7
ZDB Id:
2542938-3
SSG:
21,3
