In:
Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam, Vietnamese Journal of Food Control, National Institute for Food Control, Vol. 5, No. 3 ( 2022-09-21), p. 342-348
Abstract:
An alternative PCR method for rapid detection of Campylobacter spp. in milk powder & nbsp;was validated according to TCVN 12365-2:2018 (ISO 16140-2:2016). In this study, & nbsp;performed with paired study, validation examines the following parameters: (1) sensitivity & nbsp;(sensitivity for the alternative method: SEalt, sensitivity for the reference method: SEref, & nbsp;relative trueness: RT, false positive ratio for the alternative method: FPR), (2) relative level & nbsp;of detection (RLOD) and (3) inclusivity and exclusivity. The result showed that: sensitivity & nbsp;for the alternative method: 85.7%, sensitivity for the reference method 92.8%, relative & nbsp;trueness: 85%, false positive ratio for the alternative method: 16.7%, relative level of & nbsp;detection: 0,859 CFU/25g. For inclusitivity testing, two reference strains were detected. For & nbsp;exclusitivity testing, all of 20 strains were not detected. All the parameters tested were met & nbsp;according to TCVN 12365-2:2018 (ISO 16140-2: 2016) requirements.
Type of Medium:
Online Resource
ISSN:
2615-9252
DOI:
10.47866/2615-9252/vjfc.3953
Language:
Unknown
Publisher:
Vietnamese Journal of Food Control, National Institute for Food Control
Publication Date:
2022
