In:
The Plant Pathology Journal, Korean Society of Plant Pathology, Vol. 38, No. 5 ( 2022-10-01), p. 533-540
Abstract:
Thunberg fritillary ( 〈 i 〉 Fritillaria thunbergii 〈 /i 〉 ), a perennial used in traditional Chinese herbal medicine, is a members of the family 〈 i 〉 Liliaceae 〈 /i 〉 . The degeneration of germplasm is a severe problem in the production of 〈 i 〉 Fritillaria thunbergii 〈 /i 〉 var. 〈 i 〉 chekiangensis 〈 /i 〉 . However, no information about viral infections of 〈 i 〉 F. thunbergii 〈 /i 〉 var. 〈 i 〉 chekiangensis 〈 /i 〉 has been reported. In this study, we sequenced the small RNAs of 〈 i 〉 F. thunbergii 〈 /i 〉 var. 〈 i 〉 chekiangensis 〈 /i 〉 from leaves and bulbs, and viruses were identified using a phylogenetic analysis and BLAST search for sequence. In addition, multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) was used to rapidly detect viruses in this variety. Our study first reported that five viruses infected 〈 i 〉 F. thunbergii 〈 /i 〉 var. 〈 i 〉 chekiangensis 〈 /i 〉 . Among them, fritillary virus Y (FVY), lily mottle virus (LMoV), Thunberg fritillary mosaic virus (TFMV), and hop yellow virus (HYV) had been reported in 〈 i 〉 F. thunbergii 〈 /i 〉 , while apple stem grooving virus was first reported in the genus 〈 i 〉 Fritillaria 〈 /i 〉 . A multiplex RT-PCR method was developed to rapidly test the four viruses FVY, LMoV, TFMV, and HYV in 〈 i 〉 F. thunbergii 〈 /i 〉 var. 〈 i 〉 chekiangensis 〈 /i 〉 . Our results provide a better understanding of the infection of 〈 i 〉 F. thunbergii 〈 /i 〉 var. 〈 i 〉 chekiangensis 〈 /i 〉 by viruses and a basic reference for the better design of suitable control measures.
Type of Medium:
Online Resource
ISSN:
1598-2254
,
2093-9280
DOI:
10.5423/PPJ.OA.04.2022.0058
Language:
English
Publisher:
Korean Society of Plant Pathology
Publication Date:
2022
detail.hit.zdb_id:
2529195-6
