In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 69, No. 5-6 ( 2014-6-1), p. 237-244
Abstract:
Response surface methodology (RSM) based on Box-Behnken design (BBD) was employed to investigate the effect of ultrasonic treatment on b-carotene production by Blakeslea trispora. The optimized strategy involved exposing three-day-old mycelial cultures to ultrasonic treatment at a fixed frequency of 20 kHz, power of 491 W, treatment time of 3 min, working time of 3 s, and rest time of 5:8 s, repeated four times at a 24-h interval. Mycelium growth was not significantly promoted under ultrasonic stimulation; however, the glucose metabolism increased by about 10%, the average size of the aggregates significantly decreased, and the uptake rate of imidazole into cells was increased about 2.5-fold. After a 6-d culture, the technique produced 173 mg=L of b-carotene and 82 mg=L of lycopene, which represented an increase of nearly 40:7% and 52:7%, respectively, over the yields obtained in cultures without ultrasonic treatment
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.5560/znc.2013-0122
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
2014
detail.hit.zdb_id:
2078107-6
SSG:
12