In:
Glasnik javnog zdravlja, Centre for Evaluation in Education and Science (CEON/CEES), Vol. 96, No. 1 ( 2022), p. 18-26
Abstract:
Numerous commercial tests for SARS-CoV-2 detection have been developed in response to the current COVID-19 pandemic. Our laboratory uses tests from two manufacturers, 2019-nCoV from Sansure and Xpert Xpress SARS-CoV-2 test from Cepheid. Protocols of different manufacturers list different predefined Ct (cycle threshold) values as cut-offs for a positive test. In this study, we focus on additional verification of Ct values for borderline positive samples, to confirm the results obtained. From May to November 2021, 88,708 tests were performed, of which 7,669 were positive and 224 were borderline positive. In retested borderline positive samples, positive results were obtained in 72.3% of the cases. Percentage of negative results upon retesting was 27.7%, which is almost a third of the total number of samples analysed. Based on these results, we can conclude that special attention must be paid when interpreting borderline positive results of RT-PCR tests. In the retesting process, if possible, it is particularly important to use a test with different properties.
Type of Medium:
Online Resource
ISSN:
2812-7552
Uniform Title:
Značaj retestiranja granično pozitivnih uzoraka pacijenata na virus SARS-CoV-2 u dijagnostici oboljenja COVID-19
DOI:
10.5937/serbjph2201018G
Language:
English
Publisher:
Centre for Evaluation in Education and Science (CEON/CEES)
Publication Date:
2022
