In:
eLife, eLife Sciences Publications, Ltd, Vol. 4 ( 2015-03-17)
Abstract:
Flies look very different from humans, but both are descended from a common ancestor that existed over 600 million years ago. Some differences between animal species are due to them having different genes: stretches of DNA that contain the instructions to make proteins and other molecules. However, often differences are caused by the same or similar genes being switched on and off at different times and in different tissues in each species. The instructions that control when and where a gene is expressed are written in the sequence of DNA bases located in the regulatory region of the gene. These instructions are written in a language that is often called the ‘gene regulatory code’. This code is read and interpreted by proteins called transcription factors that bind to specific sequences of DNA (or ‘DNA words’) and increase or decrease gene expression. Changes in gene expression between species could therefore be due to changes in the transcription factors and/or changes in the instructions within the regulatory regions of specific genes. Gene regulatory regions are not well conserved between species. However, it is unclear if the instructions in these regions are written using the same gene regulatory code, and whether transcription factors found in different species recognize different DNA words. Nitta et al. have now used high-throughput methods to identify the DNA words recognized by 242 transcription factors from a fruit fly called Drosophila melanogaster. Nitta et al. then used new computational tools to find motifs, or collections of DNA words, that are recognized by each of the transcription factors. By comparing the motifs, they observed that, in spite of more than 600 million years of evolution, almost all known motifs found in humans and mice were recognized by fruit fly transcription factors. Nitta et al. noted that both fruit flies and humans have transcription factors that recognize a few unique motifs, and confer properties that are specific to each species. For example, some of the transcription factors that control the development of the fruit fly wing are not present in humans. Moreover, fruit flies lack both mucus-producing goblet cells and the ability to recognize a motif read by the transcription factor that controls the development of these cells in humans. The findings of Nitta et al. also indicate that transcription factors do not evolve to recognize subtly different DNA motifs, but instead appear constrained to recognize the same motifs. Thus, much like the genetic code that instructs how to build proteins, the gene regulatory code that determines how DNA sequences direct gene expression is also highly conserved in animals. The language used to guide the development of animals has, as such, remained very similar for millions of years. What makes animals different is differences in the content and length of the instructions that are written using this language into the regulatory regions of their genes.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.04837.001
DOI:
10.7554/eLife.04837.002
DOI:
10.7554/eLife.04837.003
DOI:
10.7554/eLife.04837.004
DOI:
10.7554/eLife.04837.005
DOI:
10.7554/eLife.04837.006
DOI:
10.7554/eLife.04837.007
DOI:
10.7554/eLife.04837.008
DOI:
10.7554/eLife.04837.009
DOI:
10.7554/eLife.04837.010
DOI:
10.7554/eLife.04837.011
DOI:
10.7554/eLife.04837.012
DOI:
10.7554/eLife.04837.013
DOI:
10.7554/eLife.04837.014
DOI:
10.7554/eLife.04837.015
DOI:
10.7554/eLife.04837.016
DOI:
10.7554/eLife.04837.017
DOI:
10.7554/eLife.04837.018
DOI:
10.7554/eLife.04837.019
DOI:
10.7554/eLife.04837.020
DOI:
10.7554/eLife.04837.021
DOI:
10.7554/eLife.04837.022
DOI:
10.7554/eLife.04837.023
DOI:
10.7554/eLife.04837.024
DOI:
10.7554/eLife.04837.025
DOI:
10.7554/eLife.04837.026
DOI:
10.7554/eLife.04837.027
DOI:
10.7554/eLife.04837.028
DOI:
10.7554/eLife.04837.029
DOI:
10.7554/eLife.04837.030
DOI:
10.7554/eLife.04837.031
DOI:
10.7554/eLife.04837.032
DOI:
10.7554/eLife.04837.033
DOI:
10.7554/eLife.04837.034
DOI:
10.7554/eLife.04837.035
DOI:
10.7554/eLife.04837.036
DOI:
10.7554/eLife.04837.037
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2015
detail.hit.zdb_id:
2687154-3
