In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-01-16)
Kurzfassung:
The centriole/basal body is a eukaryotic organelle that plays essential roles in cell division and signaling. Among five known core centriole proteins, SPD-2/Cep192 is the first recruited to the site of daughter centriole formation and regulates the centriolar localization of the other components in C. elegans and in humans. However, the molecular basis for SPD-2 centriolar localization remains unknown. Here, we describe a new centriole component, the coiled-coil protein SAS-7, as a regulator of centriole duplication, assembly and elongation. Intriguingly, our genetic data suggest that SAS-7 is required for daughter centrioles to become competent for duplication, and for mother centrioles to maintain this competence. We also show that SAS-7 binds SPD-2 and regulates SPD-2 centriolar recruitment, while SAS-7 centriolar localization is SPD-2-independent. Furthermore, pericentriolar material (PCM) formation is abnormal in sas-7 mutants, and the PCM-dependent induction of cell polarity that defines the anterior-posterior body axis frequently fails. We conclude that SAS-7 functions at the earliest step in centriole duplication yet identified and plays important roles in the orchestration of centriole and PCM assembly.
Materialart:
Online-Ressource
ISSN:
2050-084X
DOI:
10.7554/eLife.20353.001
DOI:
10.7554/eLife.20353.002
DOI:
10.7554/eLife.20353.003
DOI:
10.7554/eLife.20353.004
DOI:
10.7554/eLife.20353.005
DOI:
10.7554/eLife.20353.006
DOI:
10.7554/eLife.20353.007
DOI:
10.7554/eLife.20353.008
DOI:
10.7554/eLife.20353.009
DOI:
10.7554/eLife.20353.010
DOI:
10.7554/eLife.20353.011
DOI:
10.7554/eLife.20353.012
DOI:
10.7554/eLife.20353.013
DOI:
10.7554/eLife.20353.014
DOI:
10.7554/eLife.20353.015
DOI:
10.7554/eLife.20353.016
DOI:
10.7554/eLife.20353.017
DOI:
10.7554/eLife.20353.018
DOI:
10.7554/eLife.20353.019
DOI:
10.7554/eLife.20353.020
Sprache:
Englisch
Verlag:
eLife Sciences Publications, Ltd
Publikationsdatum:
2017
ZDB Id:
2687154-3
