In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-08-08)
Abstract:
Adhesion-type G protein-coupled receptors (aGPCRs), a large molecule family with over 30 members in humans, operate in organ development, brain function and govern immunological responses. Correspondingly, this receptor family is linked to a multitude of diverse human diseases. aGPCRs have been suggested to possess mechanosensory properties, though their mechanism of action is fully unknown. Here we show that the Drosophila aGPCR Latrophilin/dCIRL acts in mechanosensory neurons by modulating ionotropic receptor currents, the initiating step of cellular mechanosensation. This process depends on the length of the extended ectodomain and the tethered agonist of the receptor, but not on its autoproteolysis, a characteristic biochemical feature of the aGPCR family. Intracellularly, dCIRL quenches cAMP levels upon mechanical activation thereby specifically increasing the mechanosensitivity of neurons. These results provide direct evidence that the aGPCR dCIRL acts as a molecular sensor and signal transducer that detects and converts mechanical stimuli into a metabotropic response.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.28360.001
DOI:
10.7554/eLife.28360.002
DOI:
10.7554/eLife.28360.003
DOI:
10.7554/eLife.28360.004
DOI:
10.7554/eLife.28360.005
DOI:
10.7554/eLife.28360.006
DOI:
10.7554/eLife.28360.007
DOI:
10.7554/eLife.28360.008
DOI:
10.7554/eLife.28360.009
DOI:
10.7554/eLife.28360.010
DOI:
10.7554/eLife.28360.011
DOI:
10.7554/eLife.28360.012
DOI:
10.7554/eLife.28360.013
DOI:
10.7554/eLife.28360.014
DOI:
10.7554/eLife.28360.015
DOI:
10.7554/eLife.28360.016
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3