In:
eLife, eLife Sciences Publications, Ltd, Vol. 8 ( 2019-02-11)
Abstract:
The VPS13A gene is associated with the neurodegenerative disorder Chorea Acanthocytosis. It is unknown what the consequences are of impaired function of VPS13A at the subcellular level. We demonstrate that VPS13A is a peripheral membrane protein, associated with mitochondria, the endoplasmic reticulum and lipid droplets. VPS13A is localized at sites where the endoplasmic reticulum and mitochondria are in close contact. VPS13A interacts with the ER residing protein VAP-A via its FFAT domain. Interaction with mitochondria is mediated via its C-terminal domain. In VPS13A-depleted cells, ER-mitochondria contact sites are decreased, mitochondria are fragmented and mitophagy is decreased. VPS13A also localizes to lipid droplets and affects lipid droplet motility. In VPS13A-depleted mammalian cells lipid droplet numbers are increased. Our data, together with recently published data from others, indicate that VPS13A is required for establishing membrane contact sites between various organelles to enable lipid transfer required for mitochondria and lipid droplet related processes.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.43561.001
DOI:
10.7554/eLife.43561.002
DOI:
10.7554/eLife.43561.003
DOI:
10.7554/eLife.43561.004
DOI:
10.7554/eLife.43561.005
DOI:
10.7554/eLife.43561.006
DOI:
10.7554/eLife.43561.007
DOI:
10.7554/eLife.43561.008
DOI:
10.7554/eLife.43561.009
DOI:
10.7554/eLife.43561.010
DOI:
10.7554/eLife.43561.011
DOI:
10.7554/eLife.43561.012
DOI:
10.7554/eLife.43561.013
DOI:
10.7554/eLife.43561.014
DOI:
10.7554/eLife.43561.015
DOI:
10.7554/eLife.43561.016
DOI:
10.7554/eLife.43561.017
DOI:
10.7554/eLife.43561.018
DOI:
10.7554/eLife.43561.019
DOI:
10.7554/eLife.43561.020
DOI:
10.7554/eLife.43561.021
DOI:
10.7554/eLife.43561.022
DOI:
10.7554/eLife.43561.023
DOI:
10.7554/eLife.43561.024
DOI:
10.7554/eLife.43561.025
DOI:
10.7554/eLife.43561.026
DOI:
10.7554/eLife.43561.027
DOI:
10.7554/eLife.43561.028
DOI:
10.7554/eLife.43561.029
DOI:
10.7554/eLife.43561.030
DOI:
10.7554/eLife.43561.031
DOI:
10.7554/eLife.43561.032
DOI:
10.7554/eLife.43561.033
DOI:
10.7554/eLife.43561.034
DOI:
10.7554/eLife.43561.036
DOI:
10.7554/eLife.43561.037
DOI:
10.7554/eLife.43561.038
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2019
detail.hit.zdb_id:
2687154-3
