In:
eLife, eLife Sciences Publications, Ltd, Vol. 8 ( 2019-04-09)
Abstract:
HIV +Elite and Viremic controllers (EC/VCs) are able to control virus infection, perhaps because of host genetic determinants. We identified 16% (21 of 131) EC/VCs with CD4 +T cells with resistance specific to R5-tropic HIV, reversed after introduction of ccr5. R5 resistance was not observed in macrophages and depended upon the method of T cell activation. CD4 +T cells of these EC/VCs had lower ccr2 and ccr5 RNA levels, reduced CCR2 and CCR5 cell-surface expression, and decreased levels of secreted chemokines. T cells had no changes in chemokine receptor mRNA half-life but instead had lower levels of active transcription of ccr2 and ccr5, despite having more accessible chromatin by ATAC-seq. Other nearby genes were also down-regulated, over a region of ~500 kb on chromosome 3p21. This same R5 resistance phenotype was observed in family members of an index VC, also associated with ccr2/ccr5 down-regulation, suggesting that the phenotype is heritable.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.44360.001
DOI:
10.7554/eLife.44360.002
DOI:
10.7554/eLife.44360.003
DOI:
10.7554/eLife.44360.004
DOI:
10.7554/eLife.44360.005
DOI:
10.7554/eLife.44360.006
DOI:
10.7554/eLife.44360.007
DOI:
10.7554/eLife.44360.008
DOI:
10.7554/eLife.44360.009
DOI:
10.7554/eLife.44360.010
DOI:
10.7554/eLife.44360.011
DOI:
10.7554/eLife.44360.012
DOI:
10.7554/eLife.44360.013
DOI:
10.7554/eLife.44360.014
DOI:
10.7554/eLife.44360.015
DOI:
10.7554/eLife.44360.016
DOI:
10.7554/eLife.44360.017
DOI:
10.7554/eLife.44360.025
DOI:
10.7554/eLife.44360.026
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2019
detail.hit.zdb_id:
2687154-3