In:
eLife, eLife Sciences Publications, Ltd, Vol. 8 ( 2019-08-16)
Abstract:
Antibiotic resistance typically induces a fitness cost that shapes the fate of antibiotic-resistant bacterial populations. However, the cost of resistance can be mitigated by compensatory mutations elsewhere in the genome, and therefore the loss of resistance may proceed too slowly to be of practical importance. We present our study on the efficacy and phenotypic impact of compensatory evolution in Escherichia coli strains carrying multiple resistance mutations. We have demonstrated that drug-resistance frequently declines within 480 generations during exposure to an antibiotic-free environment. The extent of resistance loss was found to be generally antibiotic-specific, driven by mutations that reduce both resistance level and fitness costs of antibiotic-resistance mutations. We conclude that phenotypic reversion to the antibiotic-sensitive state can be mediated by the acquisition of additional mutations, while maintaining the original resistance mutations. Our study indicates that restricting antimicrobial usage could be a useful policy, but for certain antibiotics only.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.47088.001
DOI:
10.7554/eLife.47088.002
DOI:
10.7554/eLife.47088.003
DOI:
10.7554/eLife.47088.005
DOI:
10.7554/eLife.47088.004
DOI:
10.7554/eLife.47088.006
DOI:
10.7554/eLife.47088.007
DOI:
10.7554/eLife.47088.008
DOI:
10.7554/eLife.47088.009
DOI:
10.7554/eLife.47088.010
DOI:
10.7554/eLife.47088.012
DOI:
10.7554/eLife.47088.011
DOI:
10.7554/eLife.47088.013
DOI:
10.7554/eLife.47088.016
DOI:
10.7554/eLife.47088.014
DOI:
10.7554/eLife.47088.015
DOI:
10.7554/eLife.47088.017
DOI:
10.7554/eLife.47088.018
DOI:
10.7554/eLife.47088.019
DOI:
10.7554/eLife.47088.023
DOI:
10.7554/eLife.47088.024
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2019
detail.hit.zdb_id:
2687154-3