In:
eLife, eLife Sciences Publications, Ltd, Vol. 8 ( 2019-10-08)
Abstract:
A fundamental goal in the biological sciences is to determine how individual cells with varied gene expression profiles and diverse functional characteristics contribute to development, physiology, and disease. Here, we report a novel strategy to assess gene expression and cell physiology in single living cells. Our approach utilizes fluorescently labeled mRNA-specific anti-sense RNA probes and dsRNA-binding protein to identify the expression of specific genes in real-time at single-cell resolution via FRET. We use this technology to identify distinct myocardial subpopulations expressing the structural proteins myosin heavy chain α and myosin light chain 2a in real-time during early differentiation of human pluripotent stem cells. We combine this live-cell gene expression analysis with detailed physiologic phenotyping to capture the functional evolution of these early myocardial subpopulations during lineage specification and diversification. This live-cell mRNA imaging approach will have wide ranging application wherever heterogeneity plays an important biological role.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.49599.001
DOI:
10.7554/eLife.49599.002
DOI:
10.7554/eLife.49599.003
DOI:
10.7554/eLife.49599.004
DOI:
10.7554/eLife.49599.005
DOI:
10.7554/eLife.49599.006
DOI:
10.7554/eLife.49599.007
DOI:
10.7554/eLife.49599.008
DOI:
10.7554/eLife.49599.009
DOI:
10.7554/eLife.49599.010
DOI:
10.7554/eLife.49599.011
DOI:
10.7554/eLife.49599.012
DOI:
10.7554/eLife.49599.014
DOI:
10.7554/eLife.49599.015
DOI:
10.7554/eLife.49599.016
DOI:
10.7554/eLife.49599.017
DOI:
10.7554/eLife.49599.018
DOI:
10.7554/eLife.49599.019
DOI:
10.7554/eLife.49599.020
DOI:
10.7554/eLife.49599.021
DOI:
10.7554/eLife.49599.023
DOI:
10.7554/eLife.49599.024
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2019
detail.hit.zdb_id:
2687154-3