In:
PeerJ, PeerJ, Vol. 11 ( 2023-06-27), p. e15641-
Abstract:
MicroRNA-154-5p (miR-154-5p) plays a role in tumorigenesis in diverse human malignancies. Nevertheless, little is known about the mechanism by which miR-154-5p alters the growth and metastasis of cervical cancer. This research aimed to analyze the role of miR-154-5p in the pathology of cervical cancer in vitro and in vivo . Methods The level of miR-154-5p in human papillomavirus 16 positive cervical cancer cells was examined by real-time quantitative polymerase chain reaction. Bioinformatics predicted the downstream targets and potential functions of miR-154-5p. Furthermore, lentiviral technology was used to construct SiHa cell lines with stable up- and down-expression levels of miR-154-5p. Its differential expression effects on the progress and metastasis of cervical cancer were analyzed using cell culture and animal models. Results MiR-154-5p showed low expression in cervical cancer cells. Overexpression of miR-154-5p could markedly inhibit the proliferation, migration, and colony formation ability of SiHa cells, concomitantly leading to G1 arrest of the cell cycle, while silencing miR-154-5p triggered the opposite results. Meanwhile, overexpression of miR-154-5p restrained the growth and metastasis of cervical cancer by silencing CUL2 in vivo . Additionally, miR-154-5p reduced CUL2 level, and overexpression of CUL2 influenced the effect of miR-154-5p in cervical cancer. In conclusion, miR-154-5p restrained the growth and metastasis of cervical cancer by directly silencing CUL2.
Type of Medium:
Online Resource
ISSN:
2167-8359
DOI:
10.7717/peerj.15641/fig-1
DOI:
10.7717/peerj.15641/fig-2
DOI:
10.7717/peerj.15641/fig-3
DOI:
10.7717/peerj.15641/fig-4
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10.7717/peerj.15641/fig-5
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10.7717/peerj.15641/fig-6
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10.7717/peerj.15641/fig-7
DOI:
10.7717/peerj.15641/fig-8
DOI:
10.7717/peerj.15641/supp-1
DOI:
10.7717/peerj.15641/supp-2
DOI:
10.7717/peerj.15641/supp-3
DOI:
10.7717/peerj.15641/supp-4
Language:
English
Publisher:
PeerJ
Publication Date:
2023
detail.hit.zdb_id:
2703241-3