Format:
Online-Ressource
Content:
Abstract: The apparition of adaptive immunity in Gnathostomata correlates with the expansion of the E-protein family to encompass E2-2, HEB, and E2A. Within the family, E2-2 and HEB are more closely evolutionarily related but their concerted action in hematopoiesis remains to be explored. Here we show that the combined disruption of E2-2 and HEB results in failure to express the early lymphoid program in Common lymphoid precursors (CLPs) and a near complete block in B-cell development. In the thymus, Early T-cell progenitors (ETPs) were reduced and T-cell development perturbed, resulting in reduced CD4 T- and increased γδ T-cell numbers. In contrast, hematopoietic stem cells (HSCs), erythro-myeloid progenitors, and innate immune cells were unaffected showing that E2-2 and HEB are dispensable for the ancestral hematopoietic lineages. Taken together, this E-protein dependence suggests that the appearance of the full Gnathostomata E-protein repertoire was critical to reinforce the gene regulatory circuits that drove the emergence and expansion of the lineages constituting humoral immunity
Note:
issn: 1664-3224
Language:
English
DOI:
10.3389/fimmu.2019.00455
URN:
urn:nbn:de:bsz:25-freidok-1493938
URL:
https://doi.org/10.3389/fimmu.2019.00455
URL:
https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-1493938
URL:
https://d-nb.info/1185977317/34
