Format:
Online-Ressource
ISSN:
1521-3927
Content:
Abstract: Controlled presentation of biomolecules on synthetic substrates is an important aspect for biomaterials development. If the immobilization of multiple biomolecules is required, highly efficient orthogonal surface chemistries are needed to ensure the precision of the immobilization. In this communication, chemical vapor deposition (CVD) copolymerization is used to fabricate polymer coatings with controlled ratio of alkyne and pentafluorophenyl ester (Pfp‐ester) groups. Cyclic argine‐glycine‐aspartic acid (cRGD) adhesion peptide and epidermal growth factor (EGF) are immobilized through alkyne–azide cycloaddtion (“click” chemistry) and active ester–amine reaction, respectively. Cell studies with human umbilical vein endothelial cells (HUVEC) and A431 cell lines demonstrate the biological activity of the coimmobilized biomolecules.
In:
volume:33
In:
number:8
In:
year:2012
In:
pages:640-645
In:
extent:6
In:
Macromolecular rapid communications, Weinheim : Wiley-VCH, 1980-, 33, Heft 8 (2012), 640-645 (gesamt 6), 1521-3927
Language:
English
DOI:
10.1002/marc.201100819
URN:
urn:nbn:de:101:1-2023022206373086494754
URL:
https://doi.org/10.1002/marc.201100819
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023022206373086494754
URL:
https://d-nb.info/1281610895/34
URL:
https://doi.org/10.1002/marc.201100819