Format:
Online-Ressource
ISSN:
1460-2075
Content:
Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segregation at anaphase. Nup155 depletion inhibited accumulation of nucleoporins at the nuclear periphery, including those recruited to chromatin early in NE formation. Electron microscopy analysis revealed that Nup155 is also required for the formation of a continuous nuclear membrane in vivo and in vitro. Time‐course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs.
In:
volume:24
In:
number:20
In:
year:2005
In:
pages:3519-3531
In:
extent:13
In:
European Molecular Biology Organization, The EMBO journal, Heidelberg : EMBO Press, 1982-, 24, Heft 20 (2005), 3519-3531 (gesamt 13), 1460-2075
Language:
English
DOI:
10.1038/sj.emboj.7600825
URN:
urn:nbn:de:101:1-2023090707240964406949
URL:
https://doi.org/10.1038/sj.emboj.7600825
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023090707240964406949
URL:
https://d-nb.info/1301467847/34
URL:
https://doi.org/10.1038/sj.emboj.7600825