Umfang:
Online-Ressource
ISSN:
1522-2683
Inhalt:
Abstract: An iminodiacetic acid (IDA)‐type adsorbent is prepared at the one end of a capillary by covalently bonding IDA to the monolithic rods of macroporous poly (glycidyl methacrylate–co‐ethylene dimethacrylate). Cu (II) is later introduced to the support via the interaction with IDA. By this means, polymer monolithic immobilized metal affinity chromatography (IMAC) materials are prepared. With such a column, IMAC for on‐line concentration and capillary electrophoresis (CE) for the subsequent analysis are hyphenated for the analysis of peptides and proteins. The reproducibility of such a column has been proved good with relative standard deviations (RSDs) of dead time of less than 5% for injection‐to‐injection and 12% for column‐to‐column (n = 3). Through application on the analysis of standard peptides and real protein samples, such a technique has shown promising in proteome study.
In:
volume:26
In:
number:11
In:
year:2005
In:
pages:2172-2178
In:
extent:7
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, 26, Heft 11 (2005), 2172-2178 (gesamt 7), 1522-2683
Sprache:
Englisch
DOI:
10.1002/elps.200410377
URN:
urn:nbn:de:101:1-2023090807044624008518
URL:
https://doi.org/10.1002/elps.200410377
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023090807044624008518
URL:
https://d-nb.info/1301860077/34
URL:
https://doi.org/10.1002/elps.200410377