Format:
Online-Ressource
ISSN:
1460-2075
Content:
The roles of UvrD and Rep DNA helicases of Escherichia coli are not yet fully understood. In particular, the reason for rep uvrD double mutant lethality remains obscure. We reported earlier that mutations in recF, recO or recR genes suppress the lethality of uvrD rep, and proposed that an essential activity common to UvrD and Rep is either to participate in the removal of toxic recombination intermediates or to favour the proper progression of replication. Here, we show that UvrD, but not Rep, directly prevents homologous recombination in vivo. In addition to RecFOR, we provide evidence that RecA contributes to toxicity in the rep uvrD mutant. In vitro, UvrD dismantles the RecA nucleoprotein filament, while Rep has only a marginal activity. We conclude that UvrD and Rep do not share a common activity that is essential in vivo: while Rep appears to act at the replication stage, UvrD plays a role of RecA nucleoprotein filament remover. This activity of UvrD is similar to that of the yeast Srs2 helicase.
In:
volume:24
In:
number:1
In:
year:2005
In:
pages:180-189
In:
extent:10
In:
European Molecular Biology Organization, The EMBO journal, Heidelberg : EMBO Press, 1982-, 24, Heft 1 (2005), 180-189 (gesamt 10), 1460-2075
Language:
English
DOI:
10.1038/sj.emboj.7600485
URN:
urn:nbn:de:101:1-2023090807510090812428
URL:
https://doi.org/10.1038/sj.emboj.7600485
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023090807510090812428
URL:
https://d-nb.info/130186580X/34
URL:
https://doi.org/10.1038/sj.emboj.7600485