Format:
Online-Ressource
ISSN:
1521-4141
Content:
Abstract: Dendritic cells (DCs) are essential in antitumor immunity. In humans, three main DC subsets are defined: two types of conventional DCs (cDC1s and cDC2s) and plasmacytoid DCs (pDCs). To study DC subsets in the tumor microenvironment (TME), it is important to correctly identify them in tumor tissues. Tumor‐derived DCs are often analyzed in cell suspensions in which spatial information about DCs which can be important to determine their function within the TME is lost. Therefore, we developed the first standardized and optimized multiplex immunohistochemistry panel, simultaneously detecting cDC1s, cDC2s, and pDCs within their tissue context. We report on this panel's development, validation, and quantitative analysis. A multiplex immunohistochemistry panel consisting of CD1c, CD303, X‐C motif chemokine receptor 1, CD14, CD19, a tumor marker, and DAPI was established. The ImmuNet machine learning pipeline was trained for the detection of DC subsets. The performance of ImmuNet was compared with conventional cell phenotyping software. Ultimately, frequencies of DC subsets within several tumors were defined. In conclusion, this panel provides a method to study cDC1s, cDC2s, and pDCs in the spatial context of the TME, which supports unraveling their specific roles in antitumor immunity.
In:
day:24
In:
month:10
In:
year:2023
In:
extent:14
In:
European journal of immunology, Weinheim : Wiley-VCH, 1971-, (24.10.2023) (gesamt 14), 1521-4141
Language:
English
DOI:
10.1002/eji.202350616
URN:
urn:nbn:de:101:1-2023102515255148667190
URL:
https://doi.org/10.1002/eji.202350616
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023102515255148667190
URL:
https://d-nb.info/1307255167/34
URL:
https://doi.org/10.1002/eji.202350616