Format:
Online-Ressource
ISSN:
1522-2683
Content:
Abstract: Capillary electrophoresis with electrochemical detection using a carbon fiber electrode in conjunction with on‐capillary copper complexation was evaluated for the determination of peptides in standard and biological matrices. Peptides composed of 2—10 amino acids were investigated. A comparison was made between the responses obtained for peptides containing the oxidizable residue tyrosine and those obtained for their respective copper complexes. Electrochemical detection of non‐tyrosine‐containing peptides and a cyclic peptide was also demonstrated. A separation of leucine (Leu)‐enkephalin and five metabolites was developed and then used for the investigation of Leu‐enkephalin metabolism in plasma. The appearance of the des‐tyrosine (des‐Tyr) Leu‐enkephalin, which cannot be detected directly at a carbon electrode, was monitored using the on‐capillary complexation technique. Direct injection of the plasma sample was possible using this methodology.
In:
volume:21
In:
number:15
In:
year:2000
In:
pages:3205-3211
In:
extent:7
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, 21, Heft 15 (2000), 3205-3211 (gesamt 7), 1522-2683
Language:
English
DOI:
10.1002/1522-2683(20000901)21:15〈3205::AID-ELPS3205〉3.0.CO;2-7
URN:
urn:nbn:de:101:1-2023110204554514235817
URL:
https://doi.org/10.1002/1522-2683(20000901)21:15〈3205::AID-ELPS3205〉3.0.CO;2-7
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023110204554514235817
URL:
https://d-nb.info/1308266472/34
URL:
https://doi.org/10.1002/1522-2683(20000901)21:15〈3205::AID-ELPS3205〉3.0.CO;2-7