Format:
Online-Ressource
ISSN:
1460-2075
Content:
Pilus assembly in Caulobacter crescentus occurs during a short period of the cell cycle and pili are only present at the flagellar pole of the swarmer cell. Here we report a novel assay to visualize pili by light microscopy that led to the purification of Caulobacter pili and the isolation of a cluster of seven genes, including the major pilin subunit gene pilA. This gene cluster encodes a novel group of pilus assembly proteins. We have shown that the pilA promoter is activated late in the cell cycle and that transcription of the pilin subunit plays an important role in the timing of pilus assembly. pilA transcription is regulated by the global two‐component response regulator CtrA, which is essential for the expression of multiple cell cycle events, providing a direct link between assembly of the pilus organelle and bacterial cell cycle control.
In:
volume:19
In:
number:13
In:
year:2000
In:
pages:3223-3234
In:
extent:12
In:
European Molecular Biology Organization, The EMBO journal, Heidelberg : EMBO Press, 1982-, 19, Heft 13 (2000), 3223-3234 (gesamt 12), 1460-2075
Language:
English
DOI:
10.1093/emboj/19.13.3223
URN:
urn:nbn:de:101:1-2023110304101876256016
URL:
https://doi.org/10.1093/emboj/19.13.3223
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023110304101876256016
URL:
https://d-nb.info/1308422687/34
URL:
https://doi.org/10.1093/emboj/19.13.3223
