Format:
Online-Ressource
ISSN:
1521-4141
Content:
Abstract: The human gp200‐MR6 molecule has previously been shown to have either an antagonistic or agonistic effect on IL‐4 function, demonstrated by inhibition of IL‐4‐induced proliferation of T cells or mimicking of IL‐4‐induced maturation of epithelium, respectively. We now show that gp200‐MR6 ligation can also mimic IL‐4 and have an anti‐proliferative pro‐maturational influence within the immune system, causing up‐regulation of co‐stimulatory molecules on B lymphocytes. Biochemical analysis and cDNA cloning reveal that gp200‐MR6 belongs to the human macrophage mannose receptor family of multidomain molecules. It comprises 1722 amino acids in toto (mature protein, 1695 amino acids; signal sequence, 27 amino acids) organized into 12 external domains (an N‐terminal cysteine‐rich domain, a fibronectin type II domain and 10 C‐type carbohydrate recognition domains), a transmembrane region and a small cytoplasmic C terminus (31 amino acids) containing a single tyrosine residue (Y1679), but no obvious kinase domain. Strong amino acid sequence identity (77 %) suggests that gp200‐MR6 is the human homologue of the murine DEC‐205, indicating that this molecule has much wider functional activity than its classical endocytic role. We also show that the gp200‐MR6 molecule is closely associated with tyrosine kinase activity; the link between gp200‐MR6 and the IL‐4 receptor may therefore be via intracellular signaling pathways, with multifunctionality residing in its extracellular multidomain structure.
In:
volume:28
In:
number:12
In:
year:1998
In:
pages:4071-4083
In:
extent:13
In:
European journal of immunology, Weinheim : Wiley-VCH, 1971-, 28, Heft 12 (1998), 4071-4083 (gesamt 13), 1521-4141
Language:
English
DOI:
10.1002/(SICI)1521-4141(199812)28:12〈4071::AID-IMMU4071〉3.0.CO;2-O
URN:
urn:nbn:de:101:1-2023112603501564607967
URL:
https://doi.org/10.1002/(SICI)1521-4141(199812)28:12〈4071::AID-IMMU4071〉3.0.CO;2-O
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023112603501564607967
URL:
https://d-nb.info/1311188045/34
URL:
https://doi.org/10.1002/(SICI)1521-4141(199812)28:12〈4071::AID-IMMU4071〉3.0.CO;2-O
