Format:
Online-Ressource
ISSN:
1522-2683
Content:
Abstract: Acidic oligosaccharides derived from glycosaminoglycan heparin were separated by polyacrylamide gradient gel electrophoresis (PAGE). The gel could be visualized using Alcian Blue dye to give a pattern of highly resolved, well defined bands. The particular banding pattern obtained was the result of a heparinase catalyzed depolymerization which afforded oligosaccharide products that differed in size by one disaccharide unit. The separated oligosaccharides could be recovered prior to staining by electroelution onto a positively charged nylon membrane by a semi‐dry transfer procedure. Subsequent elution and quantitative recovery of individual oligosaccharides from the membrane was achieved. By using multiple membrane layers a second separation dimension was obtained, resulting in increased oligosaccharide purity proportional to transfer depth. Preparative gradient polyacrylamide gel electrophoresis followed by semi‐dry electro‐transfer and recovery represents a novel method for the preparation of homogeneous acidic oligosaccharides.
In:
volume:11
In:
number:1
In:
year:2005
In:
pages:23-28
In:
extent:6
In:
Electrophoresis, Weinheim : Wiley-Blackwell, 1980-, 11, Heft 1 (2005), 23-28 (gesamt 6), 1522-2683
Language:
English
DOI:
10.1002/elps.1150110106
URN:
urn:nbn:de:101:1-2024012006524288785574
URL:
https://doi.org/10.1002/elps.1150110106
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024012006524288785574
URL:
https://d-nb.info/1316692523/34
URL:
https://doi.org/10.1002/elps.1150110106