Umfang:
Online-Ressource
ISSN:
1521-4141
Inhalt:
Abstract: To further define DNA immunization as a model for anti‐DNA production, we investigated the cellular requirements for this response in mice immunized with single‐stranded DNA from E. coli. The anti‐DNA responses of genetically immune‐deficient mice and congenic controls were measured by ELISA after immunization with E. coli DNA as complexes with methylated bovine serum albumin in complete Freund's adjuvant. T cell‐deficient BALB/c‐nulnu mice failed to produce IgG anti‐DNA by this protocol despite high backgrounds of IgM anti‐DNA. In contrast, CBA/N mice expressing the xid defect displayed IgG anti‐DNA responses comparable to those of CBA/J mice despite a reduced IgM response; the specificity of CBA/N and CBA/J anti‐DNA antibodies was similar as determined by binding to synthetic DNA and RNA antigens. These results suggest that the anti‐DNA response stimulated by DNA immunization is dependent on T cells but not the B cell population affected by xid. The intact IgG response of immunized xid mice differs from that of lupus mice bearing xid where this gene defect leads to significant reduction of spontaneous anti‐DNA production.
In:
volume:20
In:
number:8
In:
year:2005
In:
pages:1789-1794
In:
extent:6
In:
European journal of immunology, Weinheim : Wiley-VCH, 1971-, 20, Heft 8 (2005), 1789-1794 (gesamt 6), 1521-4141
Sprache:
Englisch
DOI:
10.1002/eji.1830200825
URN:
urn:nbn:de:101:1-2024012407103063128468
URL:
https://doi.org/10.1002/eji.1830200825
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2024012407103063128468
URL:
https://d-nb.info/1317022173/34
URL:
https://doi.org/10.1002/eji.1830200825
