Format:
Online-Ressource
ISSN:
2391-5412
Content:
Abstract: Circulating miR-150-5p has been identified as a prognostic marker in patients with critical illness and sepsis. Herein, we aimed to further explore the role and underlying mechanism of miR-150-5p in sepsis. Quantitative real-time-PCR assay was performed to detect the expression of miR-150-5p upon stimulation with lipopolysaccharide (LPS) in RAW264.7 cells. The levels of tumor necrosis factor-α, interleukin (IL)-6 and IL-1β were measured by ELISA assay. Cell apoptosis was determined using flow cytometry. Western blot was used to assess notch receptor 1 (Notch1) expression in LPS-induced RAW264.7 cells. Dual-luciferase reporter assay was employed to validate the target of miR-150-5p. Our data showed that miR-150-5p was downregulated and Notch1 was upregulated in LPS-stimulated RAW264.7 cells. miR-150-5p overexpression or Notch1 silencing alleviated LPS-induced inflammatory response and apoptosis in RAW264.7 cells. Moreover, Notch1 was a direct target of miR-150-5p. Notch1 abated miR-150-5p-mediated anti-inflammation and anti-apoptosis in LPS-induced RAW264.7 cells. miR-150-5p alleviated LPS-induced inflammatory response and apoptosis at least partly by targeting Notch1 in RAW264.7 cells, highlighting miR-150-5p as a target in the development of anti-inflammation and anti-apoptosis drugs for sepsis treatment.
In:
volume:15
In:
number:1
In:
year:2020
In:
pages:544-552
In:
extent:9
In:
Open life sciences, Berlin : De Gruyter, 2015-, 15, Heft 1 (2020), 544-552 (gesamt 9), 2391-5412
Language:
English
DOI:
10.1515/biol-2020-0058
URN:
urn:nbn:de:101:1-2409201701506.106883565295
URL:
https://doi.org/10.1515/biol-2020-0058
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2409201701506.106883565295
URL:
https://d-nb.info/1342574702/34