Format:
1 Online-Ressource
ISSN:
1932-6203
Content:
5-Lipoxygenase (5-LO) catalyzes the two initial steps in the biosynthesis of leukotrienes (LT), a group of inflammatory lipid mediators derived from arachidonic acid. Here, we investigated the regulation of 5-LO mRNA expression by alternative splicing and nonsense-mediated mRNA decay (NMD). In the present study, we report the identification of 2 truncated transcripts and 4 novel 5-LO splice variants containing premature termination codons (PTC). The characterization of one of the splice variants, 5-LOΔ3, revealed that it is a target for NMD since knockdown of the NMD factors UPF1, UPF2 and UPF3b in the human monocytic cell line Mono Mac 6 (MM6) altered the expression of 5-LOΔ3 mRNA up to 2-fold in a cell differentiation-dependent manner suggesting that cell differentiation alters the composition or function of the NMD complex. In contrast, the mature 5-LO mRNA transcript was not affected by UPF knockdown. Thus, the data suggest that the coupling of alternative splicing and NMD is involved in the regulation of 5-LO gene expression.
In:
PLOS ONE, San Francisco, California, US : PLOS, 2006-, Band 7, Heft 2 (2012), Artikel-ID: e31363, 1932-6203
In:
volume:7
In:
year:2012
In:
number:2
In:
extent:8
In:
elocationid:e31363
Language:
English
DOI:
10.1371/journal.pone.0031363
URN:
urn:nbn:de:hebis:30:3-227807
