Format:
1 Online-Ressource (x, 288 Seiten)
ISBN:
9780511504747
Content:
A detailed technical review of label-free biosensor techniques with worked examples.
Content:
Cover -- Half-title -- Title -- Copyright -- Contents -- Contributors -- Preface -- 1 Label-free optical biosensors: An introduction -- INTRODUCTION -- RATIONALE FOR LABEL-FREE DETECTION -- OPTICAL BIOSENSORS -- PERFORMANCE METRICS -- Sensitivity -- Assay sensitivity -- Resolution (or limit of determination) -- Ease of use -- Sensor cost -- Detection instrumentation -- Throughput -- REVIEW OF OPTICAL BIOSENSOR METHODS -- Surface plasmon resonance -- HOLOGRAM BIOSENSORS -- Reflectometric interference spectroscopy -- Dual polarization interferometry -- PHOTONIC CRYSTAL BIOSENSOR -- WHISPERING GALLERY MODE RESONATORS -- ACTIVE RESONATORS: DFB LASER BIOSENSOR -- CONCLUSIONS -- REFERENCES -- 2 Experimental design -- INTRODUCTION -- ASSAY FORMAT -- IMMOBILIZATION -- Ligand activity -- Regeneration -- Regeneration scouting experiments -- Analysis of regeneration data -- SIGNAL CORRECTIONS -- Reference subtraction -- Solvent correction -- BUFFER SCOUTING -- MISCELLANEOUS -- Wash procedures -- Temperature -- Positive and negative controls -- Startup and maintenance procedures -- Software wizards -- Sample preparation -- SMALL-MOLECULE SCREENING -- PROTEIN-PROTEIN KINETICS -- General principles -- Ligand saturation -- Association data -- Dissociation data -- Ligand activity -- Systematic errors and blank injections -- Analysis order -- Reference surface -- Flow rate -- Simulation as a tool for experimental design -- ACKNOWLEDGMENTS -- REFERENCES -- 3 Extracting affinity constants from biosensor binding responses -- INTRODUCTION -- USING SPR TO OBTAIN AFFINITY CONSTANTS -- DEFINING AFFINITY CONSTANTS -- METHODS FOR DETERMINING AFFINITY CONSTANTS USING SPR -- Direct equilibrium analysis -- Generating equilibrium data -- Data processing -- Applying a simple binding model -- Applying complex binding models -- Indirect affinity determination -- SUMMARY.
Additional Edition:
9780521711517
Additional Edition:
9780521884532
Additional Edition:
Erscheint auch als Druck-Ausgabe 9780521884532
Language:
English
