Umfang:
1 online resource (585 pages)
ISBN:
9781493911363
Serie:
Advances in Plant Biology Ser. v.5
Inhalt:
Plastids are the sites of conversion of solar energy into the chemical energy usable to sustain life. They are also responsible for the production of the vast majority of the oxygen in the atmosphere. Through these activities they play a unique role in the biosphere, producing two critical products upon which life on Earth depends. It covers in 21 chapters nearly all actively investigated areas of plastid biology, from biosynthesis to function to their uses in biotechnology. The editors have compiled an extensive list of international experts from whom to solicit chapters. As is evident from the suggested Table of Contents, the book will start with a discussion of genetic material and its expression, followed by differentiation and development of different plastid types and internal organization. This is followed by an in depth look at biogenesis and assembly of plastid proteins and protein complexes and then by the important metabolic functions in plastids. The book will end with two chapters discussing the role of plastid biology in protein expression biotechnology and in hydrogen and biofuel production.
Inhalt:
Preface -- Contents -- Contributors -- Part I -- Genetic Material and its Expression -- Chapter 1 -- Chloroplast Gene Expression-RNA Synthesis and Processing -- 1.1 Introduction -- 1.2 RNA Synthesis -- 1.2.1 The Plastid-Encoded Plastid RNA Polymerase (PEP) is a Bacteria-Type Multisubunit RNA Polymerase -- 1.2.2 PEP Promoters -- 1.2.3 The Nuclear-Encoded Plastid RNA Polymerase (NEP) is Represented by Phage-Type RNA Polymerases -- 1.2.4 NEP Promoters -- 1.2.5 Division of Labor among Different Plastid RNA Polymerases -- 1.3 RNA Processing -- 1.3.1 Chloroplast RNA Splicing -- 1.3.1.1 Chloroplast Introns and Factors -- 1.3.1.2 Regulation of Chloroplast RNA Splicing -- 1.3.2 Chloroplast RNA Editing -- 1.3.2.1 Chloroplast RNA Editing Sites and Factors -- 1.3.2.2 Regulation of Chloroplast RNA Editing -- 1.3.3 RNA Cleavage and Degradation -- 1.3.3.1 Chloroplast RNases -- 1.3.3.2 Intercistronic mRNA Processing -- 1.3.3.3 Regulation of RNA Degradation -- 1.3.4 RNA Processing: Outlook -- References -- Chapter 2 -- Chloroplast Gene Expression-Translation -- 2.1 Introduction -- 2.2 Technical Considerations for Measuring Translational Activities in Chloroplasts -- 2.2.1 Genetic Approaches -- 2.2.1.1 Model Organisms -- 2.2.1.2 Chloroplast Reporter Genes -- 2.2.2 Measurement of in vivo Protein Synthesis -- 2.2.2.1 Pulse Labelling of Proteins -- 2.2.2.2 Polysomal Loading -- 2.2.3 Reconstituted Systems -- 2.2.3.1 In Vitro Translation Systems -- 2.2.3.2 RNA Binding Assays -- 2.3 Constituents of the Chloroplast Translational Apparatus -- 2.3.1 Basic Elements and Factors -- 2.3.1.1 Chloroplast tRNAs and Codon Usage -- 2.3.1.2 Chloroplast Ribosomes and General Translation Factors -- 2.3.1.3 Shine-Dalgarno Sequences and Start Codon Recognition -- 2.3.2 Gene Specific Elements and Factors for Translation Initiation -- 2.3.2.1 Cis-acting Signals on Chloroplast RNAs.
Anmerkung:
Description based on publisher supplied metadata and other sources
Weitere Ausg.:
9781493911356
Weitere Ausg.:
Erscheint auch als Druck-Ausgabe 9781493911356
Sprache:
Englisch
