UID:
almafu_9958080608302883
Umfang:
1 online resource (375 p.)
ISBN:
1-281-04635-3
,
9786611046354
,
0-08-053637-9
Serie:
Methods in Neurosciences ; Volume 31
Inhalt:
Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.Key Features* Comprehensive protocols included for* Detection of NO and related com
Anmerkung:
Description based upon print version of record.
,
Front Cover; Nitric Oxide Synthase: Characterization and Functional Analysis; Copyright Page; Table of Contents; Contributors; Preface; Volumes in Series; Section I: Enzyme Activity Measurements, Nitric Oxide Detection, and Tissue Localization; Chapter 1. Electron Paramagnetic Resonance Detection of Nitric Oxide in Biological Samples; Chapter 2. Electrochemical and Spectroscopic Methods of Nitric Oxide Detection; Chapter 3. Detection of S-Nitrosothiols; Chapter 4. Nitrosation of Proteins; Chapter 5. Immunohistochemical Methods for Nitric Oxide Synthase; Chapter 6. NADPH Diaphorase Staining
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Chapter 7. Immunohistochemical Visualization of Cyclic NucleotidesChapter 8. Detection of Downstream Components of Cyclic GMP Cascade by in Situ Hybridization Analysis; Chapter 9. Detection of Heme Oxygenase-1 and -2 Transcripts by Northern Blot and in Situ Hybridization Analysis; Section II: Enzyme Expression, Purification, and Gene Regulation; Chapter 10. Purification of Endothelial Nitric Oxide Synthase; Chapter 11. Purification of Brain Nitric Oxide Synthase from Baculovirus Overexpression System and Determination of Cofactors
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Chapter 12. Prokaryotic Expression, Purification, and Characterization of Intact and Modular Constructs of Neuronal Nitric Oxide SynthaseChapter 13. Generation of Monoclonal Antibodies to Endothelial Nitric Oxide Synthase; Chapter 14. Genetic Modulation of Inducible Nitric Oxide Synthase Expression; Chapter 15. Expression of Constitutive Brain Nitric Oxide Synthase and Inducible Nitric Oxide Synthase by Cultured Neurons and Glia; Chapter 16. Structural Characterization of Human Neuronal Nitric Oxide Synthase Gene: Methodological Approach to a Complex Transcription Unit
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Chapter 17. Transcriptional Control of Endothelial Nitric Oxide Synthase Gene ExpressionChapter 18. Site-Directed Mutagenesis to Probe Endothelial Nitric Oxide Synthase Posttranslational Modifications; Section III: Assessment of Nitric Oxide-Mediated Functions at Cell and Organ Levels; Chapter 19. Nitric Oxide and Endothelial Regulation of Vascular Tone; Chapter 20. Function of Nitric Oxide in Neuronal Cell Death; Chapter 21. Nitric Oxide-Cyclic Guanosine Monophosphate Pathway in Central Nervous System: In Vitro and in Viuo Investigations
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Chapter 22. Detection and Contribution of Nitric Oxide to Endothelium Regulation of Microvascular ToneChapter 23. Regulation of Cerebral Circulation by Nitric Oxide; Chapter 24. Nitric Oxide in NAD/NADH-Dependent Protein Modification; Chapter 25. Assessment of Synaptic Effects of Nitric Oxide in Hippocampal Neurons; Chapter 26. Synaptic Transmission in Hippocampal Slice; Chapter 27. Redox Congeners of Nitric Oxide, N-Methyl-D-Aspartate Receptors, and Intracellular Calcium Ion; Chapter 28. Cytosolic and Nuclear Calcium Imaging by Confocal Microscopy
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Chapter 29. Effects of Nitrogen Monoxide on Cellular Iron Metabolism
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English
Weitere Ausg.:
ISBN 0-12-185301-2
Sprache:
Englisch
Schlagwort(e):
Electronic books.
