UID:
almafu_9958132549102883
Format:
1 online resource (768 p.)
ISBN:
1-281-01093-6
,
9786611010935
,
0-08-049664-4
Series Statement:
Methods in enzymology ; v. 305
Content:
Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for application of chemiluminescence technology have been developed and refined, promising to further reduce the need to use radioisotopes in basic research and clinical laboratory settings. Methods for detection of low levels
Note:
Description based upon print version of record.
,
Front Cover; Bioluminescence and Chemiluminescence; Copyright Page; Table of Contents; Contributors to Volume 305; Preface; Volumes in Series; Section I: Chemiluminescence and Bioluminescence: Overviews; Chapter 1. Chemical Generation of Excited States: The Basis of Chemiluminescence and Bioluminescence; Chapter 2. Some Brief Notes on Nomenclature and Units and Standards Used in Bioluminescence and Chemiluminescence; Section II: Instrumentation; Chapter 3. Physics of Low Light Detectors; Chapter 4. Luminometer Design and Low Light Detection
,
Chapter 5. Absolute Calibration of Luminometers with Low-Level Light StandardsChapter 6. Commercially Available Luminometers and Low-Level Light Imaging Devices; Chapter 7. Automated Recordings of Bioluminescence with Special Reference to the Analysis of Circadian Rhythms; Chapter 8. Chemiluminescence Imaging Systems for the Analysis of Macrosamples: Microtiter Format, Blot Membrane, and Whole Organs; Section III: Luciferases, Luminescence Accessory Proteins, and Substrates; Chapter 9. Overexpression of Bacterial Luciferase and Purification from Recombinant Sources
,
Chapter 10. Purification of Luciferase Subunits from Recombinant SourcesChapter 11. A Rapid Chromatographic Method to Separate the Subunits of Bacterial Luciferase in Urea Containing Buffer; Chapter 12. Purification and Ligand Exchange Protocols for Antenna Proteins from Bioluminescent Bacteria; Chapter 13. Purification of Firefly Luciferase from Recombinant Sources; Chapter 14. Chemical Synthesis of Firefly Luciferin Analogs and Inhibitors; Chapter 15. Structural Basis for Understanding Spectral Variations in Green Fluorescent Protein
,
Chapter 16. Large-Scale Purification of Recombinant GFP from Escherichia coliChapter 17. Recombinant Obelin: Cloning and Expression of cDNA, Purification, and Characterization as a Calcium Indicator; Chapter 18. Gonyaulax Luciferase: Gene Structure, Protein Expression, and Purification from Recombinant Sources; Chapter 19. Dinoflagellate Luciferin-Binding Protein; Section IV: Bacterial Autoinduction System and Its Applications; Chapter 20. Assay of Autoinducer Activity with Luminescent Escherichia coli Sensor Strains Harboring a Modified Vibrio fischeri lux Regulon
,
Chapter 21. Detection, Purification, and Structural Elucidation of the Acylhomoserine Lactone Inducer of Vibrio fischeri Luminescence and Other Related MoleculesChapter 22. Chemical Synthesis of Bacterial Autoinducers and Analogs; Chapter 23. Overexpression of Foreign Proteins Using the Vibrio fischeri lux Control System; Section V: Luminescence-Based Assays in Vitro; Chapter 24. Application of Bioluminescence and Chemiluminescence in the Biomedical Sciences; Chapter 25. Use of Firefly Luciferase in ATP-Related Assays of Biomass, Enzymes, and Metabolites
,
Chapter 26. Chemiluminescent Methods for Detecting and Quantitating Enzyme Activity
,
English
Additional Edition:
ISBN 0-12-182206-0
Language:
English
