UID:
almafu_9959328724402883
Format:
1 online resource (x, 326 pages) :
,
illustrations
ISBN:
3527604375
,
9783527604371
,
3527604391
,
9783527604395
Content:
This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn: How RNAi works in worms, flies and mammals; How to design the most efficient RNAi constructs; How to achieve transient, stable and conditional RNAi in cell cultures; How to determine the efficiency of an RNAi experiment; and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiment.
Note:
Introduction : RNA interference, the "breakthrough of the year 2002" --
,
RNAi in Caenorhabditis elegans --
,
RNAi in Drosophila --
,
RNAi in mammals --
,
Suppliers of RNAi-related chemicals and probes.
Additional Edition:
Print version: Schepers, Ute. RNA interference in practice. Weinheim : Wiley-VCH, ©2005 ISBN 3527310207
Language:
English
Keywords:
Electronic books.
;
Electronic books.
;
Electronic books.
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/3527604391
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/3527604391
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/3527604391