UID:
almafu_9960073667002883
Format:
1 online resource (xv, 472 pages) :
,
illustrations (some color)
ISBN:
9780123947956
,
0123947952
Series Statement:
Gale eBooks
Content:
Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemic
Note:
Description based upon print version of record.
,
Front Cover; Proteomic and Metabolomic Approaches to Biomarker Discovery; Copyright; Contents; Preface; List of Contributors; Chapter 1 - Biomarker Discovery: Study Design and Execution; ABBREVIATIONS; INTRODUCTION; DEFINITIONS; THE CURRENT STATE OF BIOMARKER DISCOVERY; STUDY DESIGN AND EXECUTION; ERRORS IN STUDY DESIGN; ERRORS IN STUDY EXECUTION; SPECIFICITY OF PROTEINS AS BIOMARKERS; STATISTICAL DATA ANALYSIS; RECOMMENDATIONS; CONCLUDING REMARKS AND RECOMMENDATIONS; Acknowledgments; References; Chapter 2 - Proteomic and Mass Spectrometry Technologies for Biomarker Discovery; INTRODUCTION
,
PROTEIN BIOMARKER DISCOVERY AND DEVELOPMENT PIPELINEPROTEOMIC SAMPLES; PROTEIN IDENTIFICATION BY MASS SPECTROMETRY; POST-TRANSLATIONAL MODIFICATIONS AS DISEASE BIOMARKERS; PROTEIN QUANTIFICATION BY MASS SPECTROMETRY; BIOMARKER VERIFICATION; BIOMARKER VALIDATION; LIMITATIONS OF MASS SPECTROMETRY FOR PROTEIN BIOMARKER DISCOVERY; CONCLUSIONS AND FUTURE OUTLOOK: INTEGRATED BIOMARKER DISCOVERY PLATFORM; References; Chapter 3 - Tissue Sample Preparation for Proteomic Analysis; INTRODUCTION; TYPES OF TISSUES AVAILABLE FOR MS-BASED PROTEOMICS; TISSUE DISRUPTION/HOMOGENIZATION
,
EXTRACTION/SOLUBILIZATION BUFFERSCONCLUDING REMARKS; Acknowledgment; References; Chapter 4 - Sample Preparation in Global Metabolomics of Biological Fluids and Tissues; ABBREVIATIONS; INTRODUCTION; AN IDEAL SAMPLE PREPARATION METHOD FOR GLOBAL METABOLOMICS?; SAMPLE PREPARATION METHODS FOR BIOFLUIDS; TISSUE METABOLOMICS; NEW TRENDS IN SAMPLE PREPARATION FOR GLOBAL METABOLOMICS; CONCLUSIONS AND FUTURE PERSPECTIVE; Acknowledgment; References; Chapter 5 - Serum and Plasma Collection: Preanalytical Variables and Standard Operating Procedures in Biomarker Research; INTRODUCTION
,
IMPORTANCE OF PREANALYTICAL VARIABLESSTANDARD OPERATING PROCEDURES (SOPS); SAMPLE SELECTION CONSIDERATIONS; HUMAN BLOOD AND ITS COMPONENTS; OTHER BIOSAMPLES; BLOOD-BORNE PATHOGENS, UNIVERSAL PRECAUTIONS, AND SAFETY; HUMAN SUBJECT RESEARCH PROTECTIONS; CONCLUSIONS; References; Chapter 6 - Current NMR Strategies for Biomarker Discovery; INTRODUCTION: WHY NMR?; NMR HARDWARE ADVANCEMENT; SAMPLE PREPARATION FOR NMR ANALYSIS; ONE-DIMENSIONAL NMR METHODS: 1H, 13C, 31P; 2D METHODS; TARGETED METABOLIC PROFILING; HIGH-RESOLUTION MAGIC ANGLE SPINNING (HR-MAS) NMR SPECTROSCOPY
,
MAGNETIC RESONANCE SPECTROSCOPY (MRS)NMR DATA PROCESSING AND PREPARATION FOR STATISTICAL ANALYSIS; NMR METABOLITE IDENTIFICATION; FUTURE DIRECTIONS AND CONCLUSION; References; Chapter 7 - Using Data-Independent Mass Spectrometry to Extend Detectable Dynamic Range without Prior Fractionation; INTRODUCTION; PACIFIC AND QUANTIFICATION; PROTEOME PROFILING WITH PACIFIC; PERSPECTIVES; References; Chapter 8 - Gas Chromatography/Mass Spectrometry-Based Metabonomics; INTRODUCTION; GC/MS IN METABONOMICS; STRATEGIES TO ADDRESS LARGE-SCALE METABONOMIC INVESTIGATIONS; CONCLUSION AND FUTURE OUTLOOK
,
References
,
English
Additional Edition:
ISBN 9780123944467
Additional Edition:
ISBN 0123944465
Language:
English
