UID:
almahu_9947366360402882
Format:
1 online resource (551 p.)
ISBN:
1-281-05330-9
,
9786611053307
,
0-08-048938-9
Series Statement:
Methods in enzymology ; v. 354
Content:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today?truly an essential publication for researchers in all fields of life sciences.Key Features* Spectroscopic Detection of Reaction Intermediates* Isotopic and Kenetic Dete
Note:
Description based upon print version of record.
,
Cover; Table of Contents; CONTRIBUTORS TO VOLUME 354; Preface; Volumes in Series; Chapter 1. Covalent Enzyme-Substrate Compounds: Detection and Catalytic Competence; Chapter 2. Rapid Mix-Quench MALDI-TOF Mass Spectrometry for Analysis of Enzymatic Systems; Chapter 3. Pre-Steady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with On-Line Rapid-Mixing Techniques; Chapter 4. Trapping of α-Glycosidase Intermediates; Chapter 5. Trapping Covalent Intermediates on β-Glycosidases
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Chapter 6. 2-Hydroxy-6-keto-nona-2,4-diene 1,9-Dioic Acid 5,6-Hydrolase: Evidence from 18O Isotope Exchange for gem-Diol IntermediateChapter 7. Nucleoside-Diphosphate Kinase: Structural and Kinetic Analysis of Reaction Pathway and Phosphohistidine Intermediate; Chapter 8. Galactose-1-Phosphate Uridylyltransferase: Kinetics of Formation and Reaction of Uridylyl- Enzyme Intermediate inWild-Type and Specifically Mutated Uridylyltransferases; Chapter 9. Kinetic Evidence for Covalent Phosphoryl- Enzyme Intermediate in Phosphotransferase Activity of Human Red Cell Pyrimidine Nucleotidases
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Chapter 14. Transaldolase B: Trapping of Schiff Base Intermediate between Dihydroxyacetone and ε-Amino Group of Active-Site Lysine Residue by Borohydride ReductionChapter 15. T4 Endonuclease V: Use of NMR and Borohydride Trapping to Provide Evidence for Covalent Enzyme-Substrate Imine Intermediate; Chapter 16. Detection of Covalent Tetrahedral Adducts by Differential Isotope Shift 13C NMR: Acetyl- Enzyme Reaction Intermediate Formed by 3-Hydroxy-3-methylglutaryl-CoA Synthase
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Chapter 17. Detection of Intermediates in Reactions Catalyzed by PLP-Dependent Enzymes: O-Acetylserine Sulfhydrylase and Serine-Glyoxalate AminotransferaseChapter 18. Protein Tyrosine Phosphatases: X-Ray Crystallographic Observation of Cysteinyl-Phosphate Reaction Intermediate; Chapter 19. GTP:GTP Guanylyltransferase: Trapping Procedures for Detecting and Characterizing Chemical Nature of Enzyme-Nucleotide Phosphoramidate Reaction Intermediate; Chapter 20. γ-Glutamyl Thioester Intermediate in Glutaminase Reaction Catalyzed by Escherichia coli Asparagine Synthetase B
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Chapter 21. γ-Glutamyltranspeptidase and γ-Glutamyl Peptide Ligases: Fluorophosphonate and Phosphonodifluoromethyl Ketone Analogs as Probes of Tetrahedral Transition State and γ-Glutamyl- Phosphate Intermediate
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English
Additional Edition:
ISBN 0-12-182257-5
Language:
English
