UID:
almahu_9947366367402882
Umfang:
1 online resource (497 p.)
ISBN:
1-281-01143-6
,
9786611011437
,
0-08-049714-4
Serie:
Methods in enzymology, v. 376
Inhalt:
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.
Anmerkung:
Description based upon print version of record.
,
Cover; Table of Contents; Contributors to Volume 376; Preface; Volumes in Series; Section I: Biophysics, Structural Biology, and Enzymology of Chromatin Proteins; Chapter 1. Fluorescence Anisotropy Assays for Analysis of ISWI-DNA and ISWI-Nucleosome Interactions; Chapter 2. Biophysical Analysis of Specific Genomic Loci Assembled as Chromatin In Vivo; Chapter 3. Visualization and 3D Structure Determination of Defined Sequence Chromatin and Chromatin Remodeling Complexes; Chapter 4. Electron Microscopic Analysis of the RSC Chromatin Remodeling Complex
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Chapter 5. Use of Optical Trapping Techniques to Study Single-Nucleosome DynamicsChapter 6. Single-Molecule Analysis of Chromatin; Chapter 7. Biochemical and Structural Characterization of Recombinant Histone Acetyltransferase Proteins; Chapter 8. Use of Nuclear Magnetic Resonance Spectroscopy to Study Structure-Function of Bromodomains; Chapter 9. Assays for the Determination of Structure and Dynamics of the Interaction of the Chromodomain with Histone Peptides; Chapter 10. Expression, Purification, and Biophysical Studies of Chromodomain Proteins
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Chapter 11. Quantitative Assays for Characterization of the Sir2 Family of NAD+-Dependent DeacetylasesChapter 12. Selective HAT Inhibitors as Mechanistic Tools for Protein Acetylation; Chapter 13. Histone Deacetylase Inhibitors: Assays to Assess Effectiveness In Vitro and In Vivo; Section II: Immunochemical Assays of Chromatin Functions; Chapter 14. Immunochemical Analysis of Chromatin; Chapter 15. Generation and Characterization of Antibodies Directed Against Di-Modified Histones, and Comments on Antibody and Epitope Recognition
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Chapter 16. Generation and Characterization of Methyl-Lysine Histone AntibodiesChapter 17. Tips in Analyzing Antibodies Directed Against Specific Histone Tail Modifications; Chapter 18. Histone Methylation: Recognizing the Methyl Mark; Chapter 19. Analysis of Genome-Wide Histone Acetylation State and Enzyme Binding Using DNA Microarrays; Chapter 20. Use of Chromatin Immunoprecipitation Assays in Genome-Wide Location Analysis of Mammalian Transcription Factors; Chapter 21. High-Throughput Screening of Chromatin Immunoprecipitates Using CpG-Island Microarrays
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Chapter 22. Chromatin Immunoprecipitation in the Analysis of Large Chromatin Domains Across Murine Antigen Receptor LociChapter 23. The Use of Chromatin Immunoprecipitation Assays in Genome-Wide Analyses of Histone Modifications; Chapter 24. Sequential Chromatin Immunoprecipitation from Animal Tissues; Chapter 25. Immunofluorescent Staining of Polytene Chromosomes: Exploiting Genetic Tools; Chapter 26. Indirect Immunofluorescent Labeling of Drosophila Polytene Chromosomes: Visualizing Protein Interactions with Chromatin In Vivo
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Chapter 27. X-Chromosome Inactivation in Mouse Embryonic Stem Cells: Analysis of Histone Modifications and Transcriptional Activity Using Immunofluorescence and FISH
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English
Weitere Ausg.:
ISBN 0-12-182780-1
Sprache:
Englisch
