Kooperativer Bibliotheksverbund

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Format: 1 online resource (1233 p.)

Edition: 2nd ed.

ISBN: 1-281-37090-8 , 9786611370909 , 0-08-056872-6

Content: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.〈b

Note: Description based upon print version of record. , Front Cover; Bioconjugate Techniques; Copyright Page; Detailed Contents; Preface to the Second Edition; Preface to the First Edition; Acknowledgments; Health and Safety; Intellectual Property; PART I: Bioconjugate Chemistry; Chapter 1. Functional Targets; 1. Modification of Amino acids, Peptides, and Proteins; 1.1. Protein Structure and Reactivity; Amino Acids; Nucleophilic Reactions and the pI of Amino Acid Side Chains; Secondary, Tertiary, and Quaternary Structure; Prosthetic Groups, Cofactors, and Post-Translational Modifications; Protecting the Native Conformation and Activity of Proteins , Oxidation of Amino Acids in Proteins and PeptidesSolvent Accessibility of Functional Targets in Proteins; 1.2. Protein Crosslinking Methods; 2. Modification of Sugars, Polysaccharides, and Glycoconjugates; 2.1. Carbohydrate Structure and Functionality; Basic Sugar Structure; Sugar Functional Groups; Polysaccharide and Glycoconjugate Structure; 2.2. Carbohydrate and Glycan Conjugation Methods; 3. Modification of Nucleic Acids and Oligonucleotides; 3.1. Polynucleotide Structure and Functionality; Nucleotide Functional Groups; RNA and DNA Structure; 3.2. Polynucleotide Crosslinking Methods , 4. Creating Specific Functionalities4.1. Introduction of Sulfhydryl Residues (Thiolation); Modification of Amines with 2-Iminothiolane (Traut 's Reagent); Modification of Amines with SATA; Modification of Amines with SATP; Modification of Amines with SPDP; Modification of Amines with SMPT; Modification of Amines with N -Acetyl Homocysteine Thiolactone; Modification of Amines with SAMSA; Modification of Aldehydes or Ketones with AMBH; Modification of Carboxylates or Phosphates with Cystamine; Modification of Proteins with Cystamine , Modification of Nucleic Acids and Oligonucleotides with CystamineUse of Disulfide Reductants; Complete Reduction of Disulfides in Protein Molecules Using DTT; Use of DTT to Cleave Disulfide-Containing Crosslinking Agents; Ellman's Assay for the Determination of Sulfhydryls; 4.2. Introduction of Carboxylate Groups; Modification of Amines with Anhydrides; Modification of Sulfhydryls with Iodoacetate; Modification of Sulfhydryls with BMPA; Modification of Hydroxyls with Chloroacetic Acid; 4.3. Introduction of Primary Amine Groups; Modification of Carboxylates with Diamines , Modification of Sulfhydryls with N-(Β-Iodoethyl)trifluoroacetamide [Aminoethyl-8]Modification of Sulfhydryls with Ethylenimine; Modification of Sulfhydryls with 2-Bromoethylamine; Modification of Sulfhydryls with 2-Aminoethyl-2'-aminoethanethiolsulfonate; Modification of Carbohydrates with Diamines; Modification of Alkylphosphates with Diamines; Modification of Aldehydes with Ammonia or Diamines; Introduction of Arylamines on Phenolic Compounds; Amine Detection Reagents; 4.4. Introduction of Aldehyde Residues; Periodate Oxidation of Glycols and Carbohydrates , Oxidase Modification of Sugar Residues , English

Additional Edition: ISBN 0-12-370501-0

Language: English