Kooperativer Bibliotheksverbund

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Format: 1 online resource (369 p.)

ISBN: 1-281-74329-1 , 9786611743291 , 0-08-053772-3

Content: Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich.Nonmammalian Genomic Analysis: A Practical Guide covers the ""how to"" aspects of preparation, handling, cloning, and analy

Note: Description based upon print version of record. , Front Cover; Nonmammalian Genomic Analysis: A Practical Guide; Copyright Page; Contents; Contributors; Preface; Chapter 1. Introduction to Pulsed-Field Gels and Preparation and Analysis of Large DNA; I. Pulsed-Field Gel Electrophoresis (PFGE); II. Materials; III. Preparation of Nonmammalian Chromosomes; IV. Enzymatic Reactions Using DNA-Agarose Plugs; V. Southern Blotting of Pulsed-Field Gels; VI. Troubleshooting Pulsed-Field Gels; References; Chapter 2. Electrophoretic Karyotyping in Fungi; I. Introduction; II. Choice of Sample Material; III. Sample Preparation , IV. Constructing Electrophoretic KaryotypesV. Applications of Electrophoretic Karyotyping; VI. Conclusion; References; Chapter 3. Isolation and Analysis of High-Molecular-Weight DNA from Plants; I. Introduction; II. Materials; III. Procedures; IV. Pitfalls; References; Chapter 4. Generating and Using DNA Markers in Plants; I. Introduction; II. Comparison of DNA Marker Systems; III. Materials; IV. Restrictive Fragment Length Polymorphism Markers; V. Cleavable Amplified Polymorphic Sequences; VI. Random Amplified Polymorphic DNA; VII. Microsatellite Markers (Simple Sequence Repeats, SSR) , VIII. Sequence-Based Polymorphism AssaysIX. Higher Multiplex Ratio Assays: Amplified Fragment Length Polymorphism and Interrepeat Amplification; X. Choosing Appropriate Technology; References; Chapter 5. Genome Mapping of Protozoan Parasites by Linking Clones; I. Introduction; II. Strategy; III. Materials; IV. Procedures; V. General Remarks and Conclusions; References; Chapter 6. Macrorestriction Mapping and Analysis of Bacterial Genomes; I. Introduction; II. Materials; III. Topology of Bacterial Genomes; IV. Construction of Macrorestriction Maps; References , Chapter 7. Cosmid Cloning with Small GenomesI. Introduction; II. General Considerations; III. Choice of Vector; IV. Construction of the Library; V. Sorting the Library; VI. Mapping Problems; VII. Summary; References; Chapter 8. Construction of PI Artificial Chromosome (PAC) Libraries from Lower Vertebrates; I. Introduction; II. Materials; III. Protocols; IV. Summary; References; Chapter 9. The Selection of Chromosome-Specific DNA Clones from African Trypanosome Genomic Libraries; I. Introduction; II. Materials; III. Procedures; IV. Notes and Pitfalls; References , Chapter 10. Analysis of the Dictyostelium discoideum GenomeI. Introduction; II. Materials; III. Preparation and Restriction Analysis of Genomic DNA; IV. The Use of YACs in Genome Analysis; V. Restriction Enzyme-Mediated Integration (REMI)-RFLP Analysis; VI. Random Insertional Mutagenesis Using REMI; References; Chapter 11. Integrated Genome Mapping by Hybridization Techniques; I. Introduction; II. Materials; III. Procedures; IV. Conclusions; References; Index , English

Additional Edition: ISBN 0-12-101285-9

Language: English