UID:
almahu_9948025610202882
Format:
1 online resource (507 p.)
Edition:
5th ed.
ISBN:
1-281-18902-2
,
9786611189020
,
0-08-056103-9
Content:
Transcription, or the process by which DNA produces RNA, is a central aspect of gene expression. Transcription factors regulate transcription during development and in disease states. As such, it is critical for researchers to gain a good understanding of the relationship between the structure of various families of transcription factors and their function, as well as roles in human disease. Since publication of the Fourth Edition, there have been major advances, notably in the areas of chromatin remodeling and genome-scale analyses. This complete update includes all new coverage of the latest
Note:
Description based upon print version of record.
,
Front cover; Eukaryotic transcription factors; Copyright page; Contents; List of tables; About the author; Preface; Preface to the fourth edition; Preface to the third edition; Preface to the second edition; Preface to the first edition; Acknowledgements; CHAPTER 1. DNA SEQUENCES, TRANSCRIPTION FACTORS AND CHROMATIN STRUCTURE; 1.1. The importance of transcription; 1.2. Chromatin structure and its remodelling; 1.2.1. Chromatin structure and gene regulation; 1.2.2. Chromatin remodelling factors; 1.2.3. Histone modifications; 1.3. DNA sequence elements; 1.3.1. The gene promoter
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1.3.2. Sequences involved in the basic process of transcription1.3.3. Sequences involved in regulated transcription; 1.3.4. Sequences which act at a distance; 1.3.5. Negatively acting DNA sequences; 1.3.6. Interaction between factors bound at various sites; 1.4. Conclusions; References; CHAPTER 2. METHODS FOR STUDYING TRANSCRIPTION FACTORS; 2.1. Introduction; 2.2. Methods for studying DNA-protein interactions; 2.2.1. DNA mobility shift assay; 2.2.2. DNAseI footprinting assay; 2.2.3. Methylation interference assay; 2.2.4. In vivo footprinting assay
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2.3. Methods for purifying and/or cloning transcription factors2.3.1. Protein purification; 2.3.2. Gene cloning; 2.4. Use of cloned genes; 2.4.1. Domain mapping of transcription factors; 2.4.2. Determining the DNA binding specificity of an uncharacterized factor; 2.4.3. Identification of target genes for transcription factors; 2.5. Conclusions; References; CHAPTER 3. RNA POLYMERASES AND THE BASAL TRANSCRIPTIONAL COMPLEX; 3.1. RNA polymerases; 3.2. The stable transcriptional complex; 3.3. RNA polymerase I; 3.4. RNA polymerase III; 3.5. RNA polymerase II
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3.5.1. Stepwise assembly of the RNA polymerase II basal transcriptional complex3.5.2. The RNA polymerase holoenzyme; 3.6. TBP: the universal transcription factor?; 3.7. Transcriptional elongation; 3.8. Conclusions; References; CHAPTER 4. FAMILIES OF DNA BINDING TRANSCRIPTION FACTORS; 4.1. Introduction; 4.2. The homeodomain; 4.2.1. Transcription factors in Drosophila development; 4.2.2. The homeobox; 4.2.3. DNA binding by the helix-turn-helix motif in the homeobox; 4.2.4. Regulation of DNA binding specificity by interactions between different homeobox proteins
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4.2.5. Homeodomain transcription factors in other organisms4.2.6. POU proteins; 4.2.7. Pax proteins; 4.3. The two cysteine two histidine Zinc finger; 4.3.1. Transcription factors with the two cysteine two histidine finger; 4.3.2. DNA binding by the two cysteine two histidine finger; 4.4. The multi-cysteine Zinc finger; 4.4.1. Nuclear receptors; 4.4.2. DNA binding by the multi-cysteine zinc finger; 4.5. The basic DNA binding domain; 4.5.1. The leucine zipper and the basic DNA binding domain; 4.5.2. The helix-loop-helix motif and the basic DNA binding domain
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4.5.3. Dimerization of basic DNA binding domain-containing factors
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English
Additional Edition:
ISBN 0-12-437176-0
Additional Edition:
ISBN 0-12-373983-7
Language:
English
