UID:
almahu_9948025611702882
Umfang:
1 online resource (514 p.)
Ausgabe:
2d ed.
ISBN:
1-281-02938-6
,
9786611029388
,
0-08-053940-8
Inhalt:
Designed for easy use by both beginning and experienced protein crystallographers, the second edition of Practical Protein Crystallography is an essential handbook for any scientist interested in solving a protein structure. The book includes examples of actual experiments and data, electron density maps, and computer methods. This second edition has new material covering CCP4, SHELX, cryocrystallography, MAD and automated fitting.Key Features* In-depth coverage of every aspect of crystallography* Coverage of the small details that can make or break projects* Strongl
Anmerkung:
Previous ed.: 1993.
,
Front Cover; Practical Protein Crystallography; Copyright Page; Contents; Foreword To The Second Edition; Preface To The Second Edition; Acknowledgments; Chapter 1. LABORATORY TECHNIQUES; 1.1 Preparing Protein Samples; 1.2 Protein Crystal Growth; 1.3 Crystal Storage and Handling; 1.4 Crystal Soaking; 1.5 Anaerobic Crystals; Chapter 2. DATA COLLECTION TECHNIQUES; 2.1 Preparing Crystals for Data Collection; 2.2 Optical Alignment; 2.3 X-Ray Sources; 2.4 Preliminary Characterization; 2.5 Heavy-Atom Derivative Scanning with Film; 2.6 Overall Data Collection Strategy
,
2.7 Overview of Older Film Techniques2.8 Four-Circle Diffractometer Data Collection; 2.9 Area Detector Data Collection; 2.10 Image Plate Data Collection; 2.11 Synchrotron Radiation Light Sources; 2.12 Data Reduction; Chapter 3. COMPUTATIONAL TECHNIQUES; 3.1 Terminology; 3.2 Basic Computer Techniques; 3.3 Data Reduction and Statistical Analysis; 3.4 The Patterson Synthesis; 3.5 Fourier Techniques; 3.6 Isomorphous Replacement Phasing; 3.7 Molecular Replacement; 3.8 Noncrystallographic Symmetry; 3.9 Density Modification; 3.10 Multiwavelength Data with Anomalous Scattering
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3.11 Refinement of Coordinates3.12 Fitting of Maps; 3.13 Analysis of Coordinates; Chapter 4. XtalView TUTORIALS; 4.1 Installation; 4.2 Obtaining Help; 4.3 Xview; 4.4 A Typical Manual Fitting Session with Xfit; 4.5 Interfacing to Other Programs; Chapter 5. PROTEIN CRYSTALLOGRAPHY COOKBOOK; 5.1 Multiple Isomorphous Replacement; 5.2 Mutant Studies; 5.3 Substrate-Analog Example; 5.4 Molecular Replacement; 5.5 Multiwavelength Anomalous Dispersion (MAD) Phasing; Chapter 6. CRYOCRYSTALLOGRAPHY: BASIC THEORY AND METHODS; 6.1 Overview; 6.2 Theory
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6.3 Room Temperature versus Low-Temperature Crystallography6.4 Cryogenic Safety; 6.5 Equipment; 6.6 Crystal Loop Mounting Techniques; 6.7 Crystal Storage Overview; 6.8 Crystal Removal and Storage; 6.9 Crystal Handling; APPENDIX A: Crystallographic Equations in Computer Code; APPENDIX B: Useful Web Sites; Practical Protein Crystallography II Web Site; Software Web Sites; Databases; Synchrotrons; Useful Information; Heavy-Atom Information; Crystallization; X-Ray Anomalous Scattering; X-Ray Equipment Vendors; Crystallographic Associations; Index; Color Plates Section
,
English
Weitere Ausg.:
ISBN 1-4933-0155-1
Weitere Ausg.:
ISBN 0-12-486052-4
Sprache:
Englisch
