UID:
almahu_9949071184802882
Format:
1 online resource (627 p.)
ISBN:
9783527823567
,
3527823565
,
9783527823574
,
3527823573
Note:
Description based upon print version of record.
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4.3 Optimization of Peptide Hydrazide-based Native Chemical Ligation.
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Cover -- Title Page -- Copyright -- Contents -- Preface -- Chapter 1 Characterization of Protein Molecules Prepared by Total Chemical Synthesis -- 1.1 Introduction -- 1.2 Chemical Protein Synthesis -- 1.3 Comments on Characterization of Synthetic Protein Molecules -- 1.3.1 Homogeneity -- 1.3.2 Amino Acid Sequence -- 1.3.3 Chemical Analogues -- 1.3.4 Limitations of SPPS -- 1.3.5 Folding as a Purification Step -- 1.4 Summary -- References -- Chapter 2 Automated Fast Flow Peptide Synthesis -- 2.1 Introduction -- 2.2 Results -- 2.2.1 Summary -- 2.2.1.1 Mechanical Principles
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2.2.1.2 Chemical Principles -- 2.2.1.3 User Interface Principles -- 2.2.1.4 Data Analysis Method -- 2.2.1.5 Outcome -- 2.2.2 First-generation Automated Fast Flow Peptide Synthesis -- 2.2.2.1 Key Findings -- 2.2.2.2 Design of First-generation AFPS -- 2.2.2.3 Characterization of First-generation AFPS -- 2.2.3 Second-generation Automated Fast Flow Peptide Synthesis -- 2.2.3.1 Key Findings -- 2.2.3.2 Design of Second-generation AFPS -- 2.2.3.3 Characterization and Use of Second-generation AFPS -- 2.2.4 Third-generation Automated Fast Flow Peptide Synthesis -- 2.2.4.1 Key Findings
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2.2.4.2 Design of Third-generation AFPS -- 2.2.4.3 Characterization of Third-generation AFPS -- 2.2.4.4 Reagent Stability Study -- 2.2.5 Fourth-generation Automated Fast Flow Peptide Synthesis -- 2.2.5.1 Key Findings -- 2.2.5.2 Effect of Solvent on Fast Flow Synthesis -- 2.2.5.3 Design and Characterization of Fourth-generation AFPS -- 2.3 Conclusions -- Acknowledgments -- References -- Chapter 3 N,S- and N,Se-Acyl Transfer Devices in Protein Synthesis -- 3.1 Introduction -- 3.2 N,S- and N,Se-Acyl Transfer Devices: General Presentation, Reactivity and Statistical Overview of Their Utilization
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3.2.1 General Presentation of N,S- and N,Se-Acyl Transfer Devices -- 3.2.2 Relative Reactivity of N,S- and N,Se-Acyl Transfer Devices -- 3.2.3 A Statistical Overview of the Synthetic Use of N,S- and N,Se-Acyl Transfer Devices for Protein Total Chemical Synthesis -- 3.3 Preparation of SEA/SeEAoff and SEAlide Peptides -- 3.3.1 Preparation of SEA and SeEA Peptides -- 3.3.2 Preparation of SEAlide Peptides -- 3.4 Redox-controlled Assembly of Biotinylated NK1 Domain of the Hepatocyte Growth Factor (HGF) Using SEA and SeEA Chemistries
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3.5 The Total Chemical Synthesis of GM2-AP Using SEAlide-based Chemistry -- 3.6 Conclusion -- References -- Chapter 4 Chemical Synthesis of Proteins Through Native Chemical Ligation of Peptide Hydrazides -- 4.1 Introduction -- 4.2 Development of Peptide Hydrazide-based Native Chemical Ligation -- 4.2.1 Conversion of Peptide Hydrazide to Peptide Azide -- 4.2.2 Acyl Azide-based Solid-phase Peptide Synthesis -- 4.2.3 Acyl Azide-based Solution-phase Peptide Synthesis -- 4.2.4 The Transesterification of Acyl Azide -- 4.2.5 Development of Peptide Hydrazide-based Native Chemical Ligation
Additional Edition:
Print version: Brik, Ashraf Total Chemical Synthesis of Proteins Newark : John Wiley & Sons, Incorporated,c2021 ISBN 9783527346608
Language:
English
Keywords:
Electronic books.
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Electronic books.
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Electronic books.
DOI:
10.1002/9783527823567
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/9783527823567
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/9783527823567
URL:
https://onlinelibrary.wiley.com/doi/book/10.1002/9783527823567
