UID:
almahu_9949251243002882
Format:
1 online resource (XI, 316 p. 61 illus., 36 illus. in color.)
Edition:
1st ed. 2018.
ISBN:
1-4939-7343-6
Series Statement:
Methods in Molecular Biology, 1681
Content:
This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III is a valuable resource for both established and novice phage scientists.
Note:
Basic Phage Mathematics -- Analysis of Host-Takeover during SPOi Infection of Bacillus subtilis -- Practical Advice on the One-Step Growth Curve -- Iron Chloride Flocculation of Bacteriophages from Seawater -- Purification of Bacteriophages using Anion-Exchange Chromatography -- Encapsulation Strategies of Bacteriophages (Felix O1) for Oral Therapeutic Application -- Encapsulation of Listeria Phage A511 by Alginate to Improve its Thermal Stability -- Application of a Virucidal Agent to Avoid Over-Estimation of Phage Kill during Phage Decontamination Assays on Ready-to-Eat Meats -- Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes -- Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology -- Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing -- Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques using SISPA -- Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque -- Preparing cDNA Libraries from Lytic Phage Infected Cells for Whole Transcriptome Analysis by RNA-Seq -- Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis -- Annotation of Bacteriophage Genome Sequences using DNA Master: An Overview -- Phage Genome Annotation using the RAST Pipeline -- Visualization of Phage Genomic Data: Comparative Genomics and Publication-Quality Diagrams.- Transposable Bacteriophages as Genetic Tools -- Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes -- Use of RP4::mini-Mu for Gene Transfer -- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu.
Additional Edition:
ISBN 1-4939-7341-X
Language:
English
DOI:
10.1007/978-1-4939-7343-9
