UID:
almahu_9949697536002882
Format:
1 online resource (563 p.)
Edition:
1st ed.
ISBN:
1-280-59619-8
,
9786613626028
,
0-12-415966-4
Series Statement:
Methods in enzymology, v. 510
Content:
Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as ""The DNSA reducing assay for measuring cellulases,"" ""Measuring processivity"" and ""In situ cellulose detection with carbohydrate-binding modules."" Comprehensively covers the topic of cellulasesInternational board of authors
Note:
Description based upon print version of record.
,
Front Cover; Cellulases; Copyright; Contents; Contributors; Preface; Volumes in Series; Chapter 1: Measurement of endo-1,4-beta-Glucanase; 1 Introduction; 2 Purity of EG Enzymes Included in the Study; 3 Polysaccharide and Oligosaccharide Substrates; 4 Viscometric Assay of EG Activity; 4.1 Substrate preparation; 4.2 Assay of enzyme activity; 4.3 Analytical results; 5 Reducing-Sugar Assays for EG Activity; 5.1 Nelson-Somogyi reducing-sugar assay (slightly modified); 5.1.1 Preparation of reagents; 5.1.1 Assay of enzyme activity; 5.2 PAHBAH reducing-sugar assay (slightly modified)
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5.2.1 Preparation of reagents5.2.2 Assay of enzyme activity; 5.3 Analytical results; 6 Soluble Chromogenic Substrates; 6.1 Substrate preparation; 6.2 Precipitant solutions; 6.2.1 Precipitant solution A (for Azo-CMC assay); 6.2.2 Precipitant solution B (for Azo-barley glucan assay); 6.3 Assay of enzyme activity; 7 Insoluble Chromogenic Substrates; 7.1 Substrate preparation; 7.2 Assay of enzyme activity; 8 Summary; References; Chapter 2: Biomass Conversion Determined via Fluorescent Cellulose Decay Assay; 1 Introduction
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2 Correlation Between Cellulose Decay Monitored by HPLC and by Fluorescence3 Assay Development and Modeling of Response Using Statistics; 3.1 Development of plate layout; 3.2 Reduction of assay time; 3.2.1 Expressing the data showing the actual result; 3.2.2 The final model for predicted result; 3.2.3 Final proof of concept; 3.2.4 Validation of the model; 4 Final Assay Procedure; 4.1 Substrate preparation; 4.1.1 Substrate loading; 4.2 Protein determination, sample dilution, and loading of microtiter plates; 4.2.1 Control sample; 4.2.2 Assay samples; 4.2.3 Plate sealing and mixing
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4.3 Incubation and fluorescence reading of FCD assay plates4.3.1 Incubation, mixing, and reading; 4.4 Calculation of enzyme activity; 4.4.1 Calculation of results; 4.4.2 Quality control and approval of results; 4.4.3 Approval criteria; Acknowledgments; References; Chapter 3: The Analysis of Saccharification in Biomass Using an Automated High-Throughput Method; 1 Introduction; 2 Automatic Saccharification Platform; 2.1 Grinding and weighing robot; 2.2 Liquid handling robot; 3 Enzyme Characterization; 3.1 Analysis of the time course in enzyme activity; 3.2 Determination of enzyme efficiency
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3.3 Monosaccharide analysis3.4 Determination of enzyme filter paper units; 4 Concluding Remarks; Acknowledgments; References; Chapter 4: Studies of Enzymatic Cleavage of Cellulose Using Polysaccharide Analysis by Carbohydrate...; 1 Introduction; 2 The Principle of PACE; 2.1 Poly-, oligo-, and monosaccharide sample preparation; 2.2 Derivatization of poly-, oligo-, and monosaccharides with ANTS; 2.3 PACE gel preparation, running, and interpretation; 2.3.1 Preparation of PACE gels; 2.3.2 Running and analyzing the samples on PACE gels; 2.3.3 PACE gel interpretation and quantification
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2.4 Cellulolytic hydrolysis of PASC
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English
Additional Edition:
ISBN 0-12-415931-1
Language:
English
