UID:
almahu_9949697619502882
Format:
1 online resource (575 p.)
ISBN:
1-280-75162-2
,
9786610751624
,
0-08-046916-7
Series Statement:
Methods in Enzymology ; 419
Content:
This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook? and handy illustrations. The collection of protocols includes the isolation and maintenance of ste
Note:
Description based upon print version of record.
,
Front Cover; Adult Stem Cells; Copyright Page; Dedication Page; Table of Contents; Contributors to Volume 419; Preface; Foreword; Volumes in Series; Section I: Ectoderm; Chapter 1: Neural Stem Cell Isolation and Characterization; Introduction; Reagents and Instrumentation; Methods; Acknowledgments; References; Chapter 2: Neural Stem Cells and Their Manipulation; Introduction; Adult Niches for Stem Cells In Vivo; In Vitro Manipulation of NSCs; Conclusions and Projections; Sample Protocols for the Culture and Characterization of NSCs; Acknowledgments; References; Chapter 3: Retinal Stem Cells
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IntroductionMaterials and Methods; Overview; References; Chapter 4: Epithelial Skin Stem Cells; Introduction to Skin Epithelial Stem Cells; Epithelial Skin Organization and the Hair Cycle; Characteristics of Skin Epithelial Stem Cells; References; Chapter 5: Dental Pulp Stem Cells; Introduction; Identification of Dental Pulp Stem Cells; Isolation of DPSCs; Differentiation of DPSCs; References; Section II: Mesoderm; Chapter 6: Postnatal Skeletal Stem Cells; Introduction; Skeletal Stem Cells and Mesenchymal Stem Cells; Defining a Skeletal Stem Cell; Origin and Nature of Skeletal Stem Cells
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Plasticity of BMSCsRole of Skeletal Stem Cells in Disease; Molecular Engineering; Potential Use of Skeletal Stem Cells in Tissue Engineering and Regenerative Medicine; Establishment of Nonclonal Populations of BMSCs In Vitro; Establishment of Clonal Populations of BMSCs In Vitro; Determination of Colony-Forming Efficiency; In Vitro Differentiation of BMSCs; In Vivo Differentiation of BMSCs; Acknowledgments; References; Chapter 7: Hematopoietic Stem Cells; Introduction; Identification and Enrichment of HSCs; Functional Characterization of Candidate HSCs
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Long-Term Culture of Candidate HSCs and ProgenitorsAcknowledgments; References; Chapter 8: Hemangioblasts and Their Progeny; Introduction; Ex Vivo Expansion of CD133+ Cells; Hematopoietic and Endothelial Differentiation of Single Cell-Derived EGFP+ Clones; Phenotypical and Functional Studies of CD133-Derived Cells during Ex Vivo Expansion and Differentiation; Materials; Reagents; Methods; References; Chapter 9: Kidney Epithelial Cells; Introduction; Analysis of Steady State Tubular Epithelium Maintenance; Regeneration of Experimentally Injured Kidney Tubules; Extratubular Progenitor Cells
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Culture of Human Tubular Progenitor CellsReferences; Chapter 10: Ovarian Germ Cells; Introduction; Origin of Primordial Germ Cells; Formation of Human Fetal Oocytes and Primordial and Primary Follicles; Immune System-Related Cells and Oogenesis in Fetal Ovaries; Fetal Programming of Follicular Renewal during Adulthood; ""Ovary within the Ovary "" Pattern and Thy-1 Differentiation Protein; Cessation of Adult Oogenesis; Oogenesis and Follicular Renewal in Adult Ovaries; Working Hypothesis on the Role of the Gonadal Environment; Differentiation of Stem Cells and Tissue Control System Theory
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Potential Treatment of Ovarian Infertility by Producing New Primary Follicles In Vivo
,
English
Additional Edition:
ISBN 0-12-373650-1
Language:
English
