UID:
almahu_9949698023602882
Format:
1 online resource (513 p.)
Edition:
3rd ed.
ISBN:
1-281-02592-5
,
9786611025922
,
0-08-053695-6
Content:
Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology.The Third Edition of this well established book meets the needs of both newcomers to the area and experienced researchers, by providing an integrated treatment of both the production and application of monoclonal antibodies. As in previous editions, detailed and critical accounts of the theory, production, purification, fragmentation, storage and radiolabelling of monoclonal antibodies ar
Note:
Description based upon print version of record.
,
Front Cover; Monoclonal Antibodies: Principles and Practice; Copyright Page; Contents; Preface to First Edition; Preface to Second Edition; Preface to Third Edition; Georges Köhler (1946-1995); List of Abbreviations; Chapter 1. Introduction; Chapter 2. The Antibody Response; 2.1 Early History; 2.2 Structure of Antibodies; 2.3 A Typical Antibody Response; 2.4 What Distinguishes Self from Nonself?; 2.5 Effector Functions of Antibodies; 2.6 Tumour Immunity; 2.7 The Cost of the Immune System: Autoimmunity and Transplant Rejection; Chapter 3. Cellular Basis of the Immune System
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3.1 The Clonal Selection Theory3.2 Lymphocyte Development--General Aspects; 3.3 B Cells; 3.4 T Cells; 3.5 Summary--Cellular Mechanisms in Antibody Production; Chapter 4. Nature of Antigens; 4.1 What Types of Substances are Antigenic?; 4.2 Proteins that are Very Different from Self; 4.3 Proteins that are Very Similar to Self: Immune Response Genes; 4.4 Proteins that are Identical to Self: Can Self Tolerance be Broken?; 4.5 Denatured (Unfolded) Proteins; 4.6 Recognition of Short Peptides by T and B Lymphocytes; 4.7 Antibodies Against Short Peptides Sometimes Recognize the Native Protein
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4.8 Carbohydrates and Lipids4.9 Haptens: Small Molecules, Such as Drugs, Hormones and Synthetic Compounds; 4.10 Adjuvants; 4.11 Summary; Chapter 5. Antibody Structure and Function; 5.1 Structure of Antibodies; 5.2 Proteolytic Fragmentation of Immunoglobulins; 5.3 Properties of the Individual Immunoglobulin Classes; 5.4 Biosynthesis and Assembly of Immunoglobulins; 5.5 Serum Electrophoresis; Chapter 6. Genetics of Antibodies; 6.1 Immunoglobulin Allotypes; 6.2 Antibody Genes; 6.3 Sequential Rearrangement and Expression of Antibody Genes in the Developing B Cell; 6.4 RNA Processing
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6.5 Generation of Antibody Diversity6.6 Genetics of Immunoglobulin Class Switches; 6.7 Affinity Maturation and Somatic Mutation in B Cells; 6.8 Immunoglobulin Genes and B Cell Neoplasia; Chapter 7. Introduction to Monoclonal Antibodies; 7. I Myeloma (Plasmacytoma); 7.2 Monoclonal Antibodies of Predefined Specificity Produced by Hybridomas; 7.3 Differences Between Conventional and Monoclonal Serology; Chapter 8. Production of Monoclonal Antibodies; 8.1 Choice of Normal Lymphocyte Donor; 8.2 Immunization Protocol; 8.3 Choice of Myeloma Cell Line for Fusion
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8.4 Equipment Required for Fusion and Growth of Hybridomas8.5 Preparation of Spleen Cells; 8.6 Preparation of Myeloma Cells; 8.7 Preparation of HAT and HT Medium; 8.8 Fusion Protocol: Important Variables; 8.9 Early Growth; 8.10 Screening Assays; 8.11 Cloning; 8.12 Large-scale Cultures; 8.13 Storage of Hybrids in Liquid Nitrogen; 8.14 Growing of Hybridomas in Animals; 8.15 Mix-ups of Cells; 8.16 Mycoplasma Contamination; Chapter 9. Purification, Fragmentation and Isotopic Labelling of Monoclonal Antibodies; 9.1 Determination of Antibody Class; 9.2 Methods Used in Antibody Purification
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9.3 Purification of Monoclonal IgG
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English
Additional Edition:
ISBN 0-12-287023-9
Language:
English
