UID:
edoccha_9958131711002883
Format:
1 online resource (459 p.)
ISBN:
1-281-01172-X
,
9786611011727
,
0-12-352566-7
,
0-08-049663-6
Series Statement:
Methods in cell biology, v. 71
Content:
This book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.* Contains culture methodology for more than a dozen types of CNS and PNS neurons *
Note:
Description based upon print version of record.
,
Cover; Contents; Contributors; Preface; Chapter 1. Comparing the Properties of Neuronal Culture Systems: A Shopping Guide for the Cell Biologist; I. Introduction; II. Approaching Experimental Questions Using Neurons; III. Choosing a Neuronal Culture System; IV. Conclusions; References; Chapter 2. Growing and Working with Peripheral Neurons; I. Introduction; II. Methods; III. Cultures; References; Chapter 3. Dissection and Culturing of Chick Ciliary Ganglion Neurons: A System Well Suited to Synaptic Study; I. General Introduction; II. Materials; III. Methods
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IV. Variations on Dissection, Dissociation, and Culturing ThemesV. Concluding Comments; References; Chapter 4. The Culture of Chick Forebrain Neurons; I. Introduction; II. Growth and Development Characteristics; III. Isolation of Single Chick Forebrain Neurons; IV. Culture Conditions for Chick Forebrain Neurons; References; Chapter 5. Growing and Working with Spinal Motor Neurons; I. Introduction; II. Incubation of Fertilized Chicken Eggs; III. Preparation of Chick Embryo; IV. Dissection of Intact Spinal Cords and Isolationof Ventral Halves
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V. Enzymatic and Mechanical Dissociation of Intact Spinal Cords or Ventral HalvesVI. Plating Motor Neurons Spinal Cord Neurons; VII. Motor Neuron Enrichment by Density Gradient Centrifugation; VIII. Experimental Use of Motor Neuron Cultures and Spinal Cord Cultures; IX. Preparation of Solutions, Culture Dishes, Media, and Media Supplements; References; Chapter 6. Avian Purkinje Neuronal Cultures: Extrinsic Control of Morphology by Cell Type and Glutamate; I. Introduction; II. Methods and Systems; III. Applications; References; Chapter 7. Culturing Hippocampal and Cortical Neurons
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I. IntroductionII. Acquisition of Hippocampal and Cortical Neurons; III. Short-Term Culture Methods; IV. Long-Term Culture Methods; V. Summary; References; Chapter 8. Working with Xenopus Spinal Neurons in Live Cell Culture; I. Introduction; II. Neuronal Labeling; III. Culturing Xenopus Spinal Neurons; IV. Live Cell Imaging and Manipulations; V. Summary; References; Chapter 9. Culturing Neurons from the Snail Helisoma; I. Introduction; II. Maintaining Animals; III. Initial Dissection; IV. Culturing Nerve Cells; References
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Chapter 10. The Tibial-1 Pioneer Pathway. An in Vivo Model for Neuronal Outgrowth and GuidanceI. Introduction; II. Development of the Tibial-1 (Ti1) Pathway; III. Guidance of Ti1 Axons; IV. Analysis of Axon Guidance Mechanisms in Grasshopper Procedures; V. Laboratory Protocols; References; Chapter 11. Techniques to Dissect Cellular and Subcellular Function in the Drosophila Nervous System; I. Introduction; II. Background Sources of Information; III. Drosophila as a Genetic Model System for Molecular Neurobiology; IV. Biochemistry of the Drosophila Nervous System
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V. Cell Biology Techniques in the Drosophila Nervous System
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English
Additional Edition:
ISBN 0-12-352565-9
Language:
English
