UID:
almafu_9958132549202883
Umfang:
1 online resource (696 p.)
ISBN:
1-281-01094-4
,
9786611010942
,
0-08-049665-2
Serie:
Methods in enzymology ; v. 307
Inhalt:
This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications
Anmerkung:
Includes indexes.
,
Front Cover; Confocal Microscopy; Copyright Page; Table of Contents; Contributors to Volume307; Preface; Volumes in Series; Section I: Theory and Practical Considerations; Chapter 1. Theoretical Basis of Confocal Microscopy; Chapter 2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope; Section II: General Techniques; Chapter 3. Equipment for Mass Storage and Processing of Data; Chapter 4. Antifading Agents for Confocal Fluorescence Microscopy; Chapter 5. Mounting Techniques for Confocal Microscopy
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Chapter 6. Preparation of Whole Mounts and Thick Sections for Confocal MicroscopyChapter 7. Cytotomography; Chapter 8. Use of Confocal Microscopy to Investigate Cell Structure and Function; Chapter 9. Combining Laser Scanning Confocal Microscopy and Electron Microscopy; Chapter 10. Construction of Line-Scan Confocal Microscope for Physiological Recording; Section III: Measurement of Subcellular Relations and Volume Determinations; Chapter 11. Three-Dimensional Visualization of Cytoskeleton by Confocal Laser Scanning Microscopy; Chapter 12. Chromosome Spread for Confocal Microscopy
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Chapter 13. Visualization of Nuclear Pore Complex and Its Distribution by Confocal Laser Scanning MicroscopyChapter 14. Measurement of Tissue Thickness Using Confocal Microscopy; Chapter 15. Measurements of Vascular Remodeling by Confocal Microscopy; Chapter 16. Cell Volume Measurements by Fluorescence Confocal Microscopy: Theoretical and Practical Aspects; Chapter 17. Volume Measurements in Confocal Microscopy; Section IV: Imaging of Processes; Chapter 18. Quantitation of Phagocytosis by Confocal Microscopy; Chapter 19. Measurement of Secretion in Confocal Microscopy
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Chapter 20. Receptor-Ligand InternalizationChapter 21. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy; Chapter 22. Localization of Proteases and Peptide Receptors by Confocal Microscopy; Chapter 23. Observation of Microcirculatory Kinetics by Real-Time Confocal Laser Scanning Microscopy; Section V: Imaging of Ions; Chapter 24. Confocal Imaging Analysis of Intracellular Ions in Mixed Cellular Systems or in Situ Using Two Types of Confocal Microscopic Systems; Chapter 25. Confocal Ca2+ Imaging of Organelles, Cells, Tissues, and Organs
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Chapter 26. Using Confocal Microscopy and Fluorescent Indicator, 6-Methoxy-N-ethylquinolinium Iodide, to Measure Changes in Intracellular ChlorideSection VI: Imaging of Specialized Tissues; Chapter27. Measurement of Mineral Gain and Loss on Dental Enamel and Dentin Using Confocal Microscopy; Chapter 28. Characterization of Ocular Cellular and Extracellular Structures Using Confocal Microscopy and Computerized Three-Dimensional Reconstruction
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Chapter 29. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo
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English
Weitere Ausg.:
ISBN 0-12-182208-7
Sprache:
Englisch
