UID:
almafu_9961427249902883
Umfang:
1 videorecording (42 min., 39 sec.) :
,
sound, color.
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004239
Serie:
Henry Stewart talks
Inhalt:
Audio-visual presentation : Chromatin composition ; Nucleosome structure and function ; Dynamic properties inherent to nucleosomes ; Invasion of nucleosomes by gene regulatory proteins ; DNA binding proteins and enzymes ; ATP dependent remodeling factors ; Nucleosome relocation.
Anmerkung:
Retrieved April 13, 2024, from https://hstalks.com/bs/620/.
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Printable Handouts -- Navigable Slide Index -- Introduction -- DNA structure and folding -- A problem of steric hindrance -- A model for accessibility of nucleosomal DNA -- Restriction enzyme digestion assay -- Restriction enzymes access nucleosomal targets -- Exposure depends on nucleosomal distance -- Exposure depends on distance inside nucleosome -- Uncoiling vs. sliding for a mononucleosome -- Site exposure occurs without nucleosome sliding -- Accessibility inside a chain of nucleosomes -- Histone tail domains are often acetylated -- Minor effects from histone acetylation -- DNA-histone "bonds" occur every helical turn -- Any protein can bind to DNA in a nucleosome -- Site exposure and histone - DNA affinity(1) -- Site exposure and histone - DNA affinity(2) -- Invasion by collaborative competition -- Cooperativity in gene activation inside nucleosome -- FRET system analyzes nucleosome dynamics -- FRET monitors distance to the closer acceptor -- Efficient FRET from native nucleosomes -- Nucleosomal conformations in salt solution -- Histone subunits dissociate at elevated [NaCl] -- Conformational change driven by acceptor (1) -- Conformational change driven by acceptor (2) -- FRET efficiency versus cation concentration -- Two different explanations of FRET results -- Equilibrium constant vs. salt concentration -- Protein binding coupled to site exposure -- DNA constructs -- LexA binding causes conformational change (1) -- DNA constructs -- LexA binding causes conformational change (2) -- LexA binding causes conformational change (3) -- Rates of spontaneous site exposure -- Measuring the rates of site exposure -- Experimental system -- Results from stopped flow experiment -- Nucleosome dynamics analyzed by spectroscopy -- Spectroscopy with donor dye only -- Spectroscopy with donor and acceptor dyes -- Rapid spontaneous site exposure in nucleosomes -- A model for transcription through a nucleosome -- Rapid spontaneous site exposure in nucleosomes -- Bulged loop diffusion model -- Bulged loop model for nucleosome remodeling -- Summary of key messages.
Sprache:
Englisch
